A method for constructing a tumor immune checkpoint inhibitor therapeutic efficacy evaluation model based on DNA methylation profile
An immune checkpoint and methylation technology, applied in the field of bioinformatics analysis, can solve the problems of low RNA extraction content, limitation, unstable gene expression profile detection, etc., to achieve high performance and expand the possible boundaries.
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[0089] Example 2 Clinical Sample Verification
[0090] The patent of the present invention applies the above feature screening and model building methods to a pan-tumor cohort from TCGA consisting of 22 tumor types and 6381 patients. And demonstrate its superiority on this cohort (Supplementary Table 1 lists all tumor types and the number of samples included in each tumor type).
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[0092] The methylation rate (β value) of about 480,000 sites measured by the above cohort Illumina Infinium HumanMethylation450BeadChip, the gene expression profile measured by RNA-seq, the somatic mutation map obtained by genome sequencing and survival time data. The tumor mutational burden and TGF-β score of each sample were calculated according to the current definition.
[0093] On this cohort, a final feature set containing 2083 DNA methylation sites was obtained through a feature selection algorithm. Attached Table 2 exemplarily lists the methylation sites of the final feature set...
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