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Mesenchymal stem cell exosome endometrial preparation, preparation method and uterine cavity perfusion tube

A technology of mesenchymal stem cells and endometrium, applied in the field of medical devices, can solve problems such as interference, drug liquid reflux, and difficulty in achieving expected therapeutic purposes

Active Publication Date: 2021-05-07
山东美毅生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the infusion of estrogen into the uterine cavity is often used in clinical practice to adjust the thickness of the endometrium. However, there is a rapid reflux of the drug solution out of the uterine cavity, resulting in a short residence time of the drug in the uterine cavity and unable to cover the entire uterine cavity, making it difficult to achieve the desired therapeutic purpose.
Moreover, estrogen has a certain interference effect on endocrine, and there are certain side effects.

Method used

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  • Mesenchymal stem cell exosome endometrial preparation, preparation method and uterine cavity perfusion tube
  • Mesenchymal stem cell exosome endometrial preparation, preparation method and uterine cavity perfusion tube
  • Mesenchymal stem cell exosome endometrial preparation, preparation method and uterine cavity perfusion tube

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Embodiment Construction

[0031] The mesenchymal stem cell exosome endometrial preparation, preparation method and uterine cavity perfusion tube of the present invention will be described in detail below.

[0032] The endometrial preparation of mesenchymal stem cell exosomes includes mesenchymal stem cell exosomes and buffer gel, and the ratio of mesenchymal stem cell exosomes to buffer gel is 1 to 5:1, which is the weight of the buffer gel When the part is 1, the part by weight of the mesenchymal stem cell exosome is 1-5. The preparation method of the mesenchymal stem cell exosome endometrium preparation is described below.

[0033] (3) Acquisition of platelet lysate: collect the whole blood of the patient, obtain the platelet concentrate after gradient centrifugation, freeze and thaw the concentrate repeatedly, and then centrifuge to remove cell debris to obtain platelet lysate; platelet lysate growth factors and other components High content and not immunogenic;

[0034] (4) Acquisition of exosome...

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Abstract

The invention discloses a mesenchymal stem cell exosome endometrial preparation, a preparation method and a uterine cavity perfusion tube. The mesenchymal stem cell exosome endometrial preparation is used for prolonging the action time of exosome in a uterine cavity and achieving the purpose of comprehensively treating endometrial injury. The endometrial preparation comprises a mesenchymal stem cell exosome and buffer gel, and the ratio of the mesenchymal stem cell exosome to the buffer gel is (1-5): 1. The preparation method comprises the following steps of acquiring a platelet lysate, namely, collecting whole blood of a patient, carrying out gradient centrifugation to obtain a platelet concentrate, repeatedly freezing and thawing the concentrate, and centrifuging to remove cell debris to obtain the platelet lysate; obtaining the mesenchymal stem cell exosome; and preparing the mesenchymal stem cell exosome endometrial preparation, namely, fully mixing the mesenchymal stem cell exosome with the buffer gel to obtain the mesenchymal stem cell exosome endometrial preparation, and storing the mesenchymal stem cell exosome endometrial preparation at 4 DEG C. The preparation has a good anti-regurgitation effect and can be reserved on the inner wall of the uterine cavity for a long time, so that the repair effect is ensured.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to a mesenchymal stem cell exosome endometrial preparation, a preparation method and a uterine cavity perfusion tube. Background technique [0002] The successful implantation of embryos is inseparable from high-quality embryos and good endometrium. Over the past 30 years, with the continuous development of assisted reproductive technology, the embryo culture system has been optimized, the evaluation of embryo selection has been enhanced, and the implantation rate has been further improved. However, according to the latest data research, despite having high-quality embryos for transfer, endometrial damage is still a major bottleneck limiting the increase in pregnancy rate. [0003] Intrauterine perfusion can promote the proliferation of endometrium, which is more conducive to the implantation of fertilized eggs. The time of intrauterine perfusion is generally carried out a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61M31/00C12N5/0775A61D7/00
CPCA61M31/00C12N5/0662A61D7/00C12N2509/00A61M2210/1433A61M2210/005
Inventor 陈春晓陈蕾任潇宇
Owner 山东美毅生物技术有限公司
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