Collagen melanocyte compound as well as preparation method and application thereof

A technology of melanocytes and collagen, which is applied in the field of biomedicine, can solve the problems of not being suitable for large-scale transplantation, difficulty in fixing the tip, and easy loss of suspension, so as to avoid the loss of a large number of cells, easy to obtain, and simple to prepare scaffolds Effect

Inactive Publication Date: 2021-05-25
HANGZHOU SINGCLEAN MEDICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, surgical treatment methods mainly include autologous epidermal transplantation, but the limitation is that epidermal transplantation requires skin removal from normal parts, resulting in new wounds and difficult nursing care
The current clinically used cell transplantation is easy to lose the suspension and is not suitable for large-scale transplantation. In addition, it is difficult to fix at the tip

Method used

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  • Collagen melanocyte compound as well as preparation method and application thereof
  • Collagen melanocyte compound as well as preparation method and application thereof
  • Collagen melanocyte compound as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Preparation method of cross-linked collagen melanocyte complex

[0021] Cut 1g of collagen sponge into a 1cm*1cm square, put it in a 1000mL beaker, add 500mL of 90% ethanol, stir magnetically at room temperature, add 9.6g of EDC, 11.5g of NHS, and react for 48h. After the reaction, the cross-linked collagen sponge was soaked in 0.1mol / L phosphate buffer solution, stirred by magnetic force for 1 hour, rinsed with purified water, sterilized by irradiation after freeze-drying.

[0022] Cut the sterile collagen sponge into a material of uniform size: the upper surface is 1cm*1cm square, with a thickness of 5mm, and then pre-spread the cut scaffold material on the cell culture plate. Dilute 200μl of melanocytes to 1*10 5 Cells / ml were seeded on collagen scaffolds and placed in a cell culture incubator at 37°C, 5% CO 2 , cultured under saturated humidity conditions. After culturing for 2 hours, add 1ml of cell culture medium (without penicillin and streptomycin)...

Embodiment 2

[0023] Embodiment 2: the preparation method of cross-linked collagen melanocyte complex

[0024] Cut 1g of collagen sponge into a 1cm*1cm square, put it in a 1000mL beaker, add 500mL of 90% ethanol, stir magnetically at room temperature, add 9.6g of EDC, 11.5g of NHS, and react for 48h. After the reaction, the cross-linked collagen sponge was soaked in 0.1mol / L phosphate buffer solution, stirred by magnetic force for 1 hour, rinsed with purified water, sterilized by irradiation after freeze-drying.

[0025] Cut the sterile collagen sponge into a material of uniform size: the upper surface is 1cm*1cm square, with a thickness of 5mm, and then pre-spread the cut scaffold material on the cell culture plate. 400μl melanocytes were divided into 1*10 5 Cells / ml were seeded on collagen scaffolds and placed in a cell culture incubator at 37°C, 5% CO 2 , cultured under saturated humidity conditions. After culturing for 2 hours, add 1ml of cell culture medium (without penicillin and s...

Embodiment 3

[0026] Embodiment 3: the preparation method of cross-linked collagen melanocyte complex

[0027] Cut 1.5g of collagen sponge into a 1cm*1cm square, put it in a 1000mL beaker, add 500mL of 90% ethanol, stir magnetically at room temperature, add 9.6g of EDC, 11.5g of NHS, and react for 48 hours. After the reaction, the cross-linked collagen sponge was soaked in 0.1mol / L phosphate buffer solution, stirred by magnetic force for 1 hour, rinsed with purified water, sterilized by irradiation after freeze-drying.

[0028] Cut the sterile collagen sponge into a material of uniform size: the upper surface is 1cm*1cm square, with a thickness of 5mm, and then pre-spread the cut scaffold material on the cell culture plate. 400μl melanocytes were divided into 1*10 5 Cells / ml were seeded on collagen scaffolds and placed in a cell culture incubator at 37°C, 5% CO 2 , cultured under saturated humidity conditions. After culturing for 2 hours, add 1ml of cell culture medium (without penicilli...

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Abstract

The invention discloses a collagen melanocyte compound as well as a preparation method and an application thereof. The collagen material is a scaffold material which is prepared on the basis of a tissue engineering technology and has good biocompatibility, mechanical performance and degradation performance. The invention relates to preparation of a collagen scaffold and construction of a collagen melanocyte compound. The melanocytes have high cell activity and excellent melanin secretion ability on the scaffold material. The collagen melanocyte compound avoids the loss of cell suspension in the cell transplantation process, and provides a good three-dimensional growth environment for cells. The collagen melanocyte compound prepared by the invention can be used for leucoderma patients or pigment deficiency and epidermal layer injury patients.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a collagen-melanocyte complex, a preparation method and an application. Background technique [0002] Vitiligo is a common primary, localized or generalized depigmented skin and mucous membrane disease. When the skin lesions first appeared, they were one or several hypopigmented spots, which gradually developed into milky white patches with clear borders, white spots with clear borders, and normal or white hair in the white spots. All parts of the body can occur, common in the back of the fingers, wrists, forearms, face, neck and around the genitals. The disease can occur in any age group, and there is no obvious gender difference. It is usually divided into localized type, sporadic type and generalized type. It is one of the refractory diseases in dermatology that damage the patient's skin, affect the appearance, and then affect the normal life, work and social interacti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071A61L27/24A61L27/38A61L27/50A61L27/60
CPCC12N5/0626A61L27/24A61L27/3804A61L27/3895A61L27/50A61L27/60C12N2533/54A61L27/362A61L27/56A61L27/3813
Inventor 曾金麻彩丽孙伟庆黄伟
Owner HANGZHOU SINGCLEAN MEDICAL PROD
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