Small-size near-infrared II fluorescence imaging contrast agent, preparation method and application thereof

A fluorescence imaging and contrast agent technology, applied in the field of nano-biomedical imaging, can solve the problems of hindering the near-infrared second region fluorescence imaging effect, short in vivo circulation time, low fluorescence brightness, etc., achieve long in vivo circulation time, simple preparation method, The effect of improving image quality

Active Publication Date: 2021-05-28
NANJING UNIV OF POSTS & TELECOMM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this type of contrast agent has the problems of low fluorescence brightness, large size (50-200 nm), short circulation time (1-3 hours) and poor stability in the body, which seriously hinders the effect of fluorescence imaging in the second near-infrared region.

Method used

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  • Small-size near-infrared II fluorescence imaging contrast agent, preparation method and application thereof
  • Small-size near-infrared II fluorescence imaging contrast agent, preparation method and application thereof
  • Small-size near-infrared II fluorescence imaging contrast agent, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Hydrophilic polymer monomer grafted oligoethylene glycol methyl ether methacrylate polymer segment (OEGMA) repeating unit number is 5 four-arm star-shaped molecular brush polymer 50 mg dissolved in 5 ml of water for self-assembly Contrast media are available.

[0035] Such as figure 1 As shown in the transmission electron microscope image of the tested contrast agent in aqueous solution, it can be obtained that the particle diameter of the contrast agent nanoparticles is 8-10 nanometers, and they are all spherical.

[0036] Such as Figure 4 As shown, the hydrodynamic diameter of the contrast agent in the aqueous solution is tested, and the particle diameter of the contrast agent nanoparticles can be obtained as 8 nanometers.

[0037] Such as Figure 7 As shown in the absorption and emission spectrum diagram of the test contrast agent, it can be seen that its ultraviolet-near infrared absorption is in the range of 700-900 nanometers, and the emission peak is above 10...

Embodiment 2

[0041] Hydrophilic polymer monomer grafted oligoethylene glycol methyl ether methacrylate polymer segment (OEGMA) repeating unit number is 10 four-arm star-shaped molecular brush polymer 100 mg dissolved in 5 ml of water for self-assembly Contrast media are available.

[0042] Such as figure 2 As shown in the transmission electron microscope image of the test contrast agent in aqueous solution, it can be obtained that the particle size of the contrast agent nanoparticles is 10-15 nanometers, and they are all spherical.

[0043] Such as Figure 5 As shown, the hydrodynamic diameter of the contrast agent in the aqueous solution is tested, and the particle diameter of the contrast agent nanoparticles can be obtained as 11 nanometers.

[0044] Such as Figure 7 As shown in the absorption and emission spectrum diagram of the test contrast agent, it can be seen that its ultraviolet-near infrared absorption is in the range of 700-900 nanometers, and the emission peak is above 100...

Embodiment 3

[0046] Hydrophilic polymer monomer grafted oligoethylene glycol methyl ether methacrylate polymer segment (OEGMA) repeating unit number is 15 four-arm star-shaped molecular brush polymer 1000 mg dissolved in 5 ml of water for self-assembly Contrast media are available.

[0047] Such as image 3 As shown in the transmission electron microscope image of the test contrast agent in the aqueous solution, it can be obtained that the particle size of the contrast agent nanoparticles is 12-20 nanometers, and they are all spherical.

[0048] Such as Figure 6 As shown, the hydrodynamic diameter of the contrast agent in the aqueous solution is tested, and the particle diameter of the contrast agent nanoparticles can be obtained as 15 nanometers.

[0049] Such as Figure 7 As shown in the absorption and emission spectrum diagram of the test contrast agent, it can be seen that its ultraviolet-near infrared absorption is in the range of 700-900 nanometers, and the emission peak is above...

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Abstract

The invention belongs to the technical field of nano biomedical imaging, and relates to a small-size (8-50 nm) near-infrared II fluorescence imaging contrast agent, a preparation method and application thereof. The small-size contrast agent is formed by self-assembling a four-arm star-shaped molecular brush polymer in a water solution, wherein the star-shaped molecular brush polymer takes a conjugated infrared II fluorescent dye as a core, has four hydrophilic side chains of polymethacrylate oligo-polyethylene glycol ester, and can be assembled into a small-size near-infrared II fluorescence imaging contrast agent with the particle size of 8-50 nm in an aqueous solution. Compared with the existing near-infrared II fluorescence imaging contrast agent, the small-size near-infrared II fluorescence imaging contrast agent prepared by the preparation method disclosed by the invention has the advantages of excellent water solubility, small molecular size, long in-vivo blood circulation time, high tumor accumulation and good fluorescence imaging effect in a near-infrared II.

Description

technical field [0001] The invention belongs to the technical field of nano-biomedical imaging, and in particular relates to a small-sized near-infrared two-zone fluorescence imaging contrast agent and a preparation method and application thereof. Background technique [0002] Fluorescence imaging in the near-infrared window (650-1700 nm) has been widely used in biotechnology fields, such as assessment of liver function, monitoring treatment response in inflammatory arthritis, non-specific tumor targeting and image-mediated tumor therapy, etc. According to the wavelength of light, the near-infrared window can be divided into the first near-infrared region (650-1000 nanometers) and the second near-infrared region (1000-1700 nanometers). [0003] Compared with the first near-infrared fluorescence imaging, the near-infrared second-region fluorescence imaging has very low autofluorescence, light absorption and scattering characteristics in biological tissues. Therefore, near-in...

Claims

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Application Information

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IPC IPC(8): C08F120/28A61K49/00C07D513/04C09K11/06
CPCC08F120/28C07D513/04C09K11/06A61K49/0019C08F2438/03C09K2211/1007C09K2211/1011C09K2211/1085C09K2211/1092C09K2211/1416C09K2211/1425C09K2211/1483C09K2211/1458
Inventor 孙鹏飞张华范曲立陈尚钰
Owner NANJING UNIV OF POSTS & TELECOMM
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