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Method for regulating proliferation and differentiation of inner ear stem cells by using Rassf2 and application of Rassf2 in hair cell regeneration

A technology for the proliferation and differentiation of inner ear stem cells, applied in epidermal cells/skin cells, biochemical equipment and methods, applications, etc., can solve the problems of low sorting efficiency and difficulty in obtaining inner ear stem cells, and achieve accurate separation results

Pending Publication Date: 2021-06-01
SOUTHEAST UNIV
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Problems solved by technology

[0010] In order to solve the deficiencies mentioned in the above-mentioned background technology, the object of the present invention is to provide a method for Rassf2 to regulate the proliferation and differentiation of inner ear stem cells and its application in hair cell regeneration, which solves the problems of difficult acquisition and low sorting efficiency of inner ear stem cells

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  • Method for regulating proliferation and differentiation of inner ear stem cells by using Rassf2 and application of Rassf2 in hair cell regeneration
  • Method for regulating proliferation and differentiation of inner ear stem cells by using Rassf2 and application of Rassf2 in hair cell regeneration
  • Method for regulating proliferation and differentiation of inner ear stem cells by using Rassf2 and application of Rassf2 in hair cell regeneration

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[0027] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0028] The present invention studies the effects of Shh, Hippo and Rassf2 on the regeneration of inner ear hair cells from the aspects of Shh, Hippo and Rassf2 regulation. Select Lgr5-positive inner ear stem cells for culture, and then up-regulate or down-regulate the expression levels of Shh and Rassf2 in the cells through cell transfection technology, and finally regulate the Shh in tissues by virus infection in the neomycin injury model of living mouse hair cells...

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Abstract

The invention relates to the field of inner ear stem cells, in particular to a method for regulating proliferation and differentiation of inner ear stem cells through Rassf2 and application of Rassf2 in hair cell regeneration. The regulation and control method comprises the following steps: 1, detecting expression conditions of Shh or Hippo and Rassf2 in cochlea in a wild type mouse; 2, studying the effect of Shh or Hippo and Rassf2 on proliferation and differentiation of the inner ear stem cells which are separated in a flow mode in an in-vitro experiment; 3, researching the effects of Shh or Hippo and Rassf2 on a living hair cell neomycin injury model. By using the Lgr5-EGFP-CreERT2 tool mouse, the separation of the inner ear stem cells (Lgr5 positive cells) is accurate and a large amount of the inner ear stem cells (Lgr5 positive cells) can be realized, and a relatively good research premise can be achieved: the Lgr5 positive inner ear stem cells of the mouse are sorted. Besides, after the separated inner ear stem cells are subjected to balling and differentiation experiments by researching Shh, Hippo and Rassf2 in an in-vitro experiment, the specific action mechanism of Shh, Hippo and Rassf2 in regulation and control of proliferation and differentiation of the inner ear stem cells and hair cell regeneration can be deeply explored.

Description

technical field [0001] The invention relates to the field of inner ear stem cells, in particular to a method for regulating the proliferation and differentiation of inner ear stem cells by Rassf2 and its application in hair cell regeneration. Background technique [0002] Hearing is one of the most important perceptions of human beings and the basis of language communication. As the basis of language recognition and understanding, auditory perception plays an important role in human social activities. Due to the rapid development of social economy and industrialization, the increasing aging of the population, the abuse of ototoxic drugs and environmental pollution factors such as noise, the number of people suffering from different degrees of ear diseases is increasing year by year. According to WHO statistics in 2019, about 5% of the world's population, or 466 million people, suffer from disabling hearing loss, of which 34 million are children. [0003] Hearing loss and d...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6876G01N33/68C12N15/12C12N5/10
CPCC12Q1/6876G01N33/68C07K14/4702C12N5/0627C12N5/0628C12Q2600/158G01N2333/4703C12N2510/00
Inventor 柴人杰周姗张莎莎程诚高下
Owner SOUTHEAST UNIV
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