Composition and kit for methylation noninvasive early detection of gynecological tumours

A technology for early detection of gynecological tumors, applied in recombinant DNA technology, biochemical equipment and methods, and microbial determination/examination. And other issues
CN112921089AInactive Publication Date: 2021-06-08BEIJING ORIGIN-POLY BIO-TEC CO LTD
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Patent Information

Authority / Receiving Office
CN ยท China
Current Assignee / Owner
BEIJING ORIGIN-POLY BIO-TEC CO LTD
Publication Date
2021-06-08
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

The invention discloses a composition and kit for methylation noninvasive early detection of gynecological tumours. The composition comprises a target gene detection primer used for detecting methylation sites of target genes; and the target genes are PTGER4, HAND2, PAX1, HOXA9, FOXD3, OPCML, HS3ST2 and JAM3. The kit comprises a detection primer and a probe designed for the gene of the composition, a reference gene detection primer and a probe, a PCR reaction liquid and other reagents. By detecting the methylation state of the gene, noninvasive early screening can be simultaneously performed on various gynecological tumours at one time; and the method has the advantages of high detection speed, low cross contamination, final result accuracy ensured by a logistic regression algorithm and the like, and helps to realize the purposes of early discovery, early diagnosis and early treatment of malignant gynecological tumours.
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Description

technical field

[0001] The invention relates to the technical field of nucleic acid in vitro diagnosis, in particular to the application of specific gene methylation markers to the early detection and auxiliary diagnosis of gynecological tumors. Background technique

[0002] Cervical cancer, endometrial cancer and ovarian cancer are the three most common malignant tumors in gynecological tumors in my country. The incidence of cervical cancer in my country is relatively high, and the incidence of endometrial cancer is also increasing year by year in recent years. As far as the survival rate of patients is concerned, the tumor cells of endometrial cancer are not easy to spread because they are confined to the uterus, and the 5-year survival rate of early stage patients can reach 70% to 80%. The 5-year survival rate can also reach about 80%. Although the incidence of ovarian cancer is lower than that of the other two gynecological tumors, the mortality rate should not be unde...

Claims

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