Visual primer for identifying carcasses of silky fowl and black-bone silky fowl, kit and detection method therefor

A technology for silkie and black-bone chickens, applied in biochemical equipment and methods, measurement/testing of microbes, DNA/RNA fragments, etc., can solve the problem that there is no effective way to identify silky silkie and black-bone chicken carcasses and other issues, to achieve good social value, broad market application prospects, and simple operation

Active Publication Date: 2021-06-11
NANCHANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no effective method to id

Method used

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  • Visual primer for identifying carcasses of silky fowl and black-bone silky fowl, kit and detection method therefor
  • Visual primer for identifying carcasses of silky fowl and black-bone silky fowl, kit and detection method therefor
  • Visual primer for identifying carcasses of silky fowl and black-bone silky fowl, kit and detection method therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Verification of Genome Differences between Silky Chicken and Black Phoenix Chicken

[0034] Step 1: Template Preparation

[0035] (1) Tissue collection: The skin samples of 24 silky chickens and 24 black-bone chickens were collected.

[0036] (2) DNA template preparation: about 50 mg of skin samples were collected, put into a centrifuge tube with 500 μL of liquid nitrogen, and the collected samples were ground with a tissue grinder. After the liquid nitrogen was evaporated to dryness, 200 microliters of TE was added, centrifuged at 1200 rpm / min, and the supernatant was extracted for later use.

[0037] Step 2: PCR Amplification

[0038] (1) PCR amplification reaction system

[0039] PCR amplification primers are shown in Table 1:

[0040] Table 1 PCR amplification primers

[0041]

[0042] The PCR reaction system is shown in Table 2:

[0043] Table 2 PCR amplification system

[0044] Reagent Volume (ul) 2x reaction buffer 12.5 ...

Embodiment 2

[0048] Reliability of Embodiment 2 Single-blind Detection and Judgment Method

[0049] In this example, a single-blind method is adopted to determine the source of the species of the samples of known species according to the molecular detection results.

[0050] Step 1: Template Preparation

[0051] (1) Tissue collection: The skin samples of 12 silky chickens and 12 black-bone chickens were collected.

[0052] (2) DNA template preparation: about 50 mg of skin samples were collected, put into a centrifuge tube with 500 μL of liquid nitrogen, and the collected samples were ground with a tissue grinder. After the liquid nitrogen was evaporated to dryness, 200 microliters of TE was added, centrifuged at 1200 rpm / min, and the supernatant was extracted for later use.

[0053] Step 2: PCR Amplification

[0054] (1) PCR amplification reaction system

[0055] PCR amplification primers are shown in Table 3:

[0056] Table 3 PCR amplification primers

[0057]

[0058] The PCR re...

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PUM

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Abstract

The invention discloses a visual primer for identifying carcasses of silky fowl and black-bone silky fowl, a kit and a detection method therefor. The primer comprises FIP: FIP, wherein a nucleotide sequence as shown in SEQ ID No. 1, BIP, wherein a nucleotide sequence is as shown in SEQ ID No. 2; F3, wherein a nucleotide sequence is as shown in SEQ ID No.3; B3: wherein a nucleotide sequence is as shown in SEQ ID No.4; the designed visual primer for identifying the silky fowl and the black-phoenix chicken can perform specific PCR amplification on the genome DNA of the silky fowl, cannot amplify the genome DNA of the black-phoenix chicken and can be used for identifying carcasses of the silky fowl and the black-phoenix chicken, after the reaction is completed, if a reaction system is sky blue, the chicken breed to be detected is the silky fowl, and if the reaction system is light purple, the chicken breed to be detected is a black boned chicken with black feather. the silky-feather black-bone chicken and the black-bone chicken carcasses can be accurately judged by 100%.

Description

technical field [0001] The invention relates to the field of molecular biology technology, in particular to a primer, a kit and a detection method for visually identifying carcasses of silky chickens and black-bone chickens. Background technique [0002] Silky chicken with silk feathers is a special product of Taihe County, Jiangxi Province. It is native to the northern foot of Wushan Mountain in Taihe County. It is famous for its ten characteristics of "claw, black skin, black meat, and black bone" as well as its high nutritional value and medicinal value. Silkie chicken is a famous food and medicine chicken. The whole body can be used as medicine. The bones, meat and viscera have medicinal value, and can be formulated into various patent medicines and prescriptions. [0003] However, the original silky-bone chicken grows slowly and has low egg production, so commercial breeding companies use silky-feather silky and fast-growing breeds as breeding materials to breed black ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/6888C12Q1/686C12Q2563/173
Inventor 许继国熊信威周敏朱学农崔芳芳马荆鄂谭玉文许桥贡继尚杨艳北李袁飞盛文涛饶友生王樟凤
Owner NANCHANG NORMAL UNIV
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