Method for identifying peripheral blood circulation endometrial cells and application

A technology of peripheral blood circulation and endometrium, which is applied in the field of cell identification, can solve problems such as false positives, and achieve the effect of improving the accuracy of identification

Active Publication Date: 2021-06-11
PEOPLES HOSPITAL PEKING UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problems existing in the prior art, after further research, the inventor found that there were false positives in the detection rate of endometriosis based on CEC in the previous study, and the reason for the false positives was mainly in the separation and detection of CEC. endothelial cells

Method used

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  • Method for identifying peripheral blood circulation endometrial cells and application
  • Method for identifying peripheral blood circulation endometrial cells and application
  • Method for identifying peripheral blood circulation endometrial cells and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0051] 1. Main experimental materials and reagents

[0052] 1. Blood samples were collected from patients undergoing surgery for pelvic masses found on ultrasound examination.

[0053] Inclusion criteria:

[0054] 1) Women of childbearing age are highly suspected of having endometriosis

[0055] 2) Prepare for surgery

[0056] Exclusion criteria:

[0057] 1) Pregnant or breastfeeding, or postmenopausal women at the time of testing

[0058] 2) Diagnosed with other malignant tumors or with a history of tumors

[0059] 3) Diagnosed with blood diseases or abnormal white blood cells

[0060] 4) Used steroid hormone drugs (including oral contraceptives, subcutaneous implanted contraceptives, progesterone intrauterine release devices, etc.) in the 3 months before the test

[0061] 5) Did not sign the informed consent.

[0062] In addition, asymptomatic women with regular menstruation and no abnormalities in ultrasonography were recruited as healthy controls.

[0063] 2. Exper...

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PUM

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Abstract

The invention discloses a method for identifying peripheral blood circulation endometrial cells and application. The identification method according to the present invention comprises a step of selecting, as candidate cells, PECAM1-, DAPI +, and PTPRC-from an enriched liquid phase obtained by removing erythrocytes and leukocytes from peripheral blood from a subject, cells in which endometrial markers are positive. According to the method, the identification accuracy of the circulating endometrial cells can be greatly improved.

Description

technical field [0001] The invention relates to the field of cell identification, in particular to a method and application for identifying peripheral blood circulating endometrium cells. Background technique [0002] Endometriosis (endometriosis, EM) has an incidence rate of more than 10% in women of reproductive age, often causing pelvic pain or infertility, and has the characteristics of easy recurrence and malignant transformation, which will not only cause physical pain, but also Bring obstacles to the patient's psychology and society. At present, the pathogenesis of EM has not yet been clarified. Sampson's menstrual flow retrograde implantation theory is generally accepted, but it cannot explain why about 90% of women have menstrual blood retrograde phenomenon, while only a part of them suffer from EM. In recent years, domestic scholars have put forward the "eutopic endometrium determinism", which believes that the abnormality of the eutopic endometrium determines the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/58G01N21/64
CPCG01N33/56966G01N33/582G01N21/6428G01N21/6458G01N2021/6439G01N2800/364
Inventor 昌晓红祝洪澜王上程洪艳叶雪
Owner PEOPLES HOSPITAL PEKING UNIV
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