Microscope with LED illumination assembly

Abstract

A microscope system is disclosed, the system comprising a multi-channel LED lighting array comprising one or more optical redirection nodes, the optical redirection nodes comprising a front face, a rear face, a top face, a bottom face, and two side faces; one or more light emitting diodes, each positioned to face one of the front face, rear face, top face, or bottom face of one of the one or more optical redirection nodes; and one or more collimating lenses, each positioned between one of the one or more light emitting diodes and one of the one or more optical redirection nodes; and a microscope assembly comprising an objective, one or more magnifying lenses, an optical path, and a specimen stage.

Description

technical field [0001] The present invention relates generally to microscopy and spectrophotometry systems, and more particularly to fluorescence imaging systems. Background technique [0002] Fluorescence microscopy enables the study of materials that can fluoresce in their natural form (known as intrinsic or autofluorescence) or when treated with chemicals capable of fluorescing (known as secondary fluorescence). Fluorescence microscopy and fluorescence microscopy are generally considered important tools in cell biology. [0003] Fluorescence occurs when a sample is illuminated with light of a certain wavelength. The fluorescence generated by the sample usually has a longer wavelength than the excitation light. This phenomenon is called Stokes shift (or photoinduced glow). Shorter wavelength light in the UV portion of the spectrum will tend to have more photon energy than longer wavelength infrared light (when considering the particle nature of light). For example, when...

AI Technical Summary

Problems solved by technology

The time interval between the absorption of the excitation light and the emission of the re-radiated light in fluorescence is usually finite but very short in duration, usually less than a millionth of a second

Images