Feed additive for promoting fish intestinal micro-ecological restoration, preparation method and application

A technology of feed additives and intestinal microecology, which is applied in the field of feed additives to promote the recovery of fish intestinal microecology, can solve the problem of no animal intestinal content, and achieve the improvement of fish intestinal structure damage and intestinal bacteria Colon disorders, accelerated intestinal mucosal repair, and improved immunity

Active Publication Date: 2021-06-18
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, in aquaculture, there is no relevant application of animal intestinal contents as feed additives

Method used

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  • Feed additive for promoting fish intestinal micro-ecological restoration, preparation method and application
  • Feed additive for promoting fish intestinal micro-ecological restoration, preparation method and application
  • Feed additive for promoting fish intestinal micro-ecological restoration, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Analysis of the composition and structure of the intestinal content of healthy koi carps, comprising the following steps:

[0039]First of all, take 9 healthy Koi carps that have no ornamental value. After koi were anesthetized with MS-222, the body surface was wiped with 75% ethanol, and the abdominal cavity was cut open from the anus along the abdomen with ophthalmic scissors. ℃ pre-cooled) Petri dish, the intestinal surface fat, blood and excess tissue were cleaned.

[0040] The intestinal contents of each fish were squeezed out and collected, then the intestinal tract was dissected and the intestinal mucosa was scraped. The intestinal contents of each 3 fish were combined into one sample, put into a 50mL centrifuge tube, mixed evenly, and stored at -80°C for later use.

[0041] Intestinal microbial genomic DNA was extracted by conventional methods, detected by 1% agarose gel electrophoresis, and then 16S rDNA high-throughput sequencing. The PCR amplifie...

Embodiment 2

[0042] Embodiment 2 The preparation method and application of the feed additive that promotes the recovery of fish intestinal microecology

[0043] Its preparation method comprises the following steps:

[0044] First, healthy koi carp with no ornamental value were used as intestinal content donors. After koi were anesthetized with MS-222, the body surface was wiped with 75% ethanol, and the abdominal cavity was cut open from the anus along the abdomen with ophthalmic scissors. ℃ pre-cooled) Petri dish, the intestinal surface fat, blood and excess tissue were cleaned.

[0045] Subsequently, intestinal tissues were transferred to a clean bench, and the contents were extruded into 50 mL centrifuge tubes. Homogenize with a homogenizer at 4°C and 50-100rpm for 30s-1.5min (preferably 100rpm for 1min). Move the homogenized intestinal contents to an anaerobic incubator as soon as possible, then dilute the homogenate with a volume ratio of homogenate:PBS of 2:1 and mix well.

[004...

Embodiment 3

[0049] Example 3 Effects of Antibiotics (Florfenicol) on Koi Intestinal Mucosa Structure

[0050] (1) Antibiotic (containing florfenicol) feed preparation: Accurately weigh the florfenicol dry powder, select the dosage according to the recommended dosage in the instructions, add it to the common feed, mix it and make it into granules in the feeder Pellet feed with a diameter of 3mm, air-dried, packed in sealed bags, and stored in a cool place.

[0051] (2) Experimental grouping and feeding: the koi were randomly divided into two groups, the control group was fed with common feed (CG-7), and the treatment group was fed with florfenicol-containing feed (FL). Sampling, 11 koi carps fed with normal feed and 11 koi with florfenicol feed were collected, the intestinal contents were separated, and the intestinal tissues were preserved. During the duration of the breeding experiment, the fish were fed twice a day, and the daily feeding amount was 2% of the fish body weight.

[0052]...

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Abstract

The invention discloses a feed additive for promoting fish intestinal micro-ecological restoration, a preparation method and application, and belongs to the technical field of microorganism application. The feed additive comprises intestinal contents separated from fish intestinal tissues, and the thallus content of the intestinal contents is 1 * 10<10>-1 * 10<12>/mL. The application of the feed additive comprises any one of the following: 1) repairing fish intestinal structure damage caused by antibiotics; and 2) improving the structural composition of the fish intestinal flora. According to the invention, the feed additive is prepared by extracting intestinal contents from fish intestinal tissues, the fish additive is added into a fish feed to improve the fish intestinal structure damage and intestinal flora disorder caused by use of antibiotics, the immunity of fishes can be improved, the use of antibiotics can be reduced, and sustainable development of aquaculture or ornamental fishery is realized.

Description

technical field [0001] The invention relates to the technical field of microbial application, and relates to a feed additive for promoting the recovery of fish intestinal microecology, a preparation method and an application. Background technique [0002] In recent years, the rapid development of my country's aquaculture industry has produced huge economic and social benefits. However, with the continuous expansion of aquaculture scale, the frequent occurrence of diseases caused by various pathogens poses a huge threat to the sustainable development of aquaculture. At present, the use of antibiotics to prevent and control fish bacterial diseases is still the main way to prevent and control them, but long-term use will lead to problems such as drug residues and pathogenic bacteria developing drug resistance. Not only that, the use of antibiotics will also significantly affect the composition and diversity of intestinal microorganisms, leading to intestinal microecological di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/26A23K10/18A23K20/105A01K61/10A01K61/13
CPCA23K50/80A23K10/26A23K10/18A23K20/105A01K61/10A01K61/13Y02A40/81
Inventor 孙敬锋韩卓然毛晴孙学亮
Owner TIANJIN AGRICULTURE COLLEGE
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