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Subculture stage calculation device, subculture stage calculation method, and program

A time-changing technology, applied in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve the problem of inability to judge whether the colony is mature enough

Pending Publication Date: 2021-06-18
NIKON CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the device described in Patent Document 1, the cells are separated from the culture container and collected in the recovery bag, and then the number of cells is measured and adjusted to perform subculture. Therefore, it is impossible to determine whether the colony has matured sufficiently, and if it is assumed to be insufficient, it cannot continue. colony maturation

Method used

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  • Subculture stage calculation device, subculture stage calculation method, and program
  • Subculture stage calculation device, subculture stage calculation method, and program
  • Subculture stage calculation device, subculture stage calculation method, and program

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no. 1 approach

[0023] Below, while referring to the attached Figure 1 Embodiments of the present invention will be described in detail. figure 1 It is a figure which shows an example of the structure of the passage time calculation apparatus 1 of this embodiment. The passage time calculation device 1 extracts a region to be extracted, that is, an extraction target region, from a plurality of phase difference images PS (not shown) in which pluripotent stem cells are captured. Here, pluripotent stem cells refer to stem cells that potentially have the ability to differentiate into all the cell series belonging to the three germ layers of endoderm, mesoderm, and ectoderm (pluripotency) and also have proliferative properties. For example, iPS cells, embryogenic stem cells (ES cells).

[0024] The plurality of phase difference images PS refers to a group of time-lapse images of colonies of pluripotent stem cells taken at predetermined time intervals, and one of the phase difference images PS is...

no. 2 approach

[0115] Below, while referring to the attached Figure 1 Next, a second embodiment of the present invention will be described in detail.

[0116] In the above-mentioned first embodiment, the case where the passage time calculation device calculates the passage time based on the decrease rate of the rib region ratio A and a predetermined threshold value has been described. In this embodiment, a case will be described in which the passage period calculation device calculates the passage period based on passage period information in which change points and passage periods are previously associated with each type of pluripotent stem cells.

[0117] The passage time calculation device of this embodiment is referred to as a passage time calculation device 1a.

[0118] Figure 6 It is a figure which shows an example of the structure of the passage time calculation apparatus 1a of this embodiment. Comparing the passage time calculation device 1a of this embodiment ( Figure 6 ) and...

no. 3 approach

[0130] Below, while referring to the attached Figure 1 Next, a third embodiment of the present invention will be described in detail.

[0131] In the above-mentioned first and second embodiments, the case where the passage time calculation device repeatedly continues the process of calculating the passage time for a plurality of frames of phase difference images has been described. In this embodiment, a case will be described in which the passage time calculation device stops the processing after calculating the passage time. In addition, in this embodiment, a case where the passage time calculation device changes the time interval at which pluripotent stem cells are imaged will be described.

[0132] The passage time calculation device of this embodiment is referred to as a passage time calculation device 1b.

[0133] Figure 8 It is a figure which shows an example of the structure of the passage time calculation apparatus 1b of this embodiment. Comparing the passage tim...

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Abstract

A subculture stage calculation device is provided with: a time change-calculating section for calculating, from multiple microscope images of pluripotent stem cells taken at different times, time changes in the area occupied by an extracted region, which is a region in which a striated pattern has appeared; a change point-detecting section for detecting a change point in time changes of the area occupied by the extracted region; and a subculture stage-calculating section for determining the subculture stage of the pluripotent stem cells on the basis of said change point.

Description

technical field [0001] The present invention relates to a passage period calculation device, a passage period calculation method and a program. [0002] This application claims priority based on Japanese Patent Application No. 2018-193875 filed in Japan on October 12, 2018, the contents of which are incorporated herein by reference. Background technique [0003] In regenerative medicine and the like, a large number of pluripotent stem cells are required. The process of increasing the number of cells of interest is called the expanded culture process, but in the expanded culture of pluripotent stem cells such as artificial pluripotent stem cells (iPS cells), it is necessary to maintain the pluripotency of the pluripotent stem cells, that is, undifferentiated cells. The state is not changed and the expansion is carried out. Therefore, in cells that form colonies such as iPS cells, the colonies are subcultured to expand culture. iPS cells, that is, pluripotent stem cells, ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34
CPCC12M41/36C12M41/48G06V20/69C12M31/10C12M33/00C12M41/46C12N5/0696
Inventor 檀知朗纪伊宏昭鱼住孝之佐藤美子吉田信介
Owner NIKON CORP
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