A method for enriching exosomes in a biological sample solution

Abstract

The invention relates to a method for enriching exosomes in a biological sample solution by using an exosome enrichment material. The material for enriching exosomes is a polymer microsphere material with a porous surface structure prepared by phase inversion technology. The material can use the principle of surface aperture sieving to effectively intercept exosomes and remove interfering substances such as proteins through internal adsorption materials. It can be applied to the enrichment of exosomes in plasma, urine, cell culture fluid, etc.

Description

A method for enrichment of exosomes in biological sample solution technical field The present invention belongs to the material for exosome enrichment and preparation method thereof and realizes exosome enrichment in different body fluids The application of the set, specifically, a material with a core as a protein adsorption material and a porous structure as the outer shell is used for exocytosis in body fluids. body enrichment. Background technique Exosome is a kind of membranous vesicle body secreted by cell, diameter is usually 30-150nm, contains protein, DNA, RNA and many other important substances. It has various functions in intercellular communication, signal transduction, disease diagnosis and treatment, and drug delivery. very important role. Widely present in plasma, urine, cell culture fluid and other substances. Therefore, to develop a fast, high It is very important to efficiently enrich high-purity exosomes [1] . For the enrichment of exoso...

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Problems solved by technology

The polymer-dependent sedimentation method is to use the polymer and exosomes to form a network and centrifuge at a relatively low speed to obtain samples. The processing method is simple, but the purity of the obtained exosomes is low, and there are many impurities such as impurities. interference

The immunoaffinity method uses specific protein-binding antibodies existing on the surface of exosomes to achieve the separation of exosomes. The specificity is better, but the difference in exosomes makes it impossible to complete the recovery of exosomes, and this method The cost is high, and it is difficult to achieve high-throughput processing

The size exclusion method is a method of separating exosomes by size, which is relatively simple and easy to implement, but the purity of the obtained exosomes is low and the interference is serious

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