Method for evaluating shade-tolerant lodging resistance of soybeans by measuring stem epidermis cell hydrogen ion flow

Abstract

The invention is suitable for the technical field of plant shade-tolerant lodging resistance evaluation, and provides a method for evaluating the shade-tolerant lodging resistance of soybeans by measuring stem epidermal cell hydrogen ion flow. The method comprises the following steps: in an original soybean growth environment, grinding soybean stem epidermis to form a ground part; adopting an equilibrium buffer solution to balance epidermal cell ion exchange of the polished part; measuring an epidermal cell hydrogen ion flow of the polished part; and carrying out shade tolerance and lodging resistance analysis and evaluation according to a hydrogen ion flow measurement result. According to the method, in the original growth environment of plants, the flow condition of hydrogen ions on the epidermis of the soybean stalks in normal illumination and shading environments is measured through a non-invasive micro-measurement technology, so that the relative strength of shade tolerance and lodging resistance of different soybean varieties is judged, and the method is simple, rapid, high in accuracy and easy to popularize. The shade-tolerant lodging resistance of the soybeans can be simply and effectively judged indoors.

Description

technical field [0001] The invention belongs to the technical field of plant shade-tolerance and lodging resistance evaluation, in particular to a method for evaluating the shade-tolerance and lodging resistance of soybean by measuring the hydrogen ion flow of stem epidermal cells. Background technique [0002] Soybean (Glycine max) originates in China and is an important source of edible vegetable protein and oil in the world. In recent years, with the improvement of people's living standards, my country's demand for soybeans has risen rapidly, and the contradiction between supply and demand has become increasingly prominent. Increasing soybean production has become an urgent need to ensure national food security. Close planting and intercropping are important means to increase soybean yield and effectively use planting space. However, mutual shading between plants will cause significant changes in the surrounding light environment, resulting in obvious shade avoidance resp...

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Problems solved by technology

[0004] To sum up, at present, soybean shade tolerance is mainly evaluated from the aspects of seedling stage characteristics, yield and yield components, and stalk lignocellulose content. The above evaluation methods all need to plant soybeans in the field, and the research process is complicated and difficult. The operation and research cycle is long; the shade-tolerant and lodging-resistant soybean materials cannot be quickly and accurately identified under indoor conditions; the real growth conditions of the plants under shade conditions cannot be reflected; and the dynamic detection of living biological materials cannot be realized. cause waste

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