Compositions of azadirachta indica and methods of treating cancer
A composition and cancer technology, applied in the direction of drug combination, non-living material medical raw materials, drug delivery, etc., can solve the problems of unscalable industrial production, harmful changes in extraction temperature and separation temperature of plant components, and infeasibility of separation temperature
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Embodiment 1
[0196] Example 1: Growth Inhibition of Oral Squamous Cell Carcinoma by Neem (Nem) Extract in Vivo: Tumor Reduction in Oral Squamous Cell Carcinoma by Disrupting the Inflammatory Cascade by Treatment with Natural Neem Leaf Extract occurrence and volume.
[0197]The leaves and bark of the neem tree (Neem) have been used for centuries in traditional Ayurvedic medicine to treat oral diseases. The experiments described herein tested the hypothesis that the initiation and / or progression of OSCC could be prevented using this neem leaf extract. The anticancer potential of neem leaf extract has been tested in in vitro and in vivo platforms. OSCC cell lines (SCC4, Cal27, HSC3) were treated with the leaf extract at different time points and markers of inflammation, invasion and proliferation were analyzed simultaneously. The preventive effect of SCNE was also evaluated in a heterotopic xenograft mouse model and a carcinogen-induced oral cancer mouse model. Treatment with neem leaf ext...
Embodiment 2
[0222] Example 2: Supercritical CO of Neem Leaves (Nem) 2 The extract and its bioactive limonoid, azadirachtin, inhibit colon cancer in preclinical models by modulating pro-inflammatory pathways.
[0223] To explore the role of neem in CRC, human colon cancer cell lines HCT116 and HT29 cells were treated with purified supercritical neem extract (SCNE) or azadirachtin. SCNE treatment showed dose-dependent inhibition of CRC cell proliferation and increased apoptosis. It was found that both SCNE and azadirachtin treatments showed decreased protein expression due to poor nuclear localization of p65, transcription factor phosphorylation STAT3, and pro-inflammatory cytokines COX1, COX2, IL-6, and TNF-α in CRC cells. Anti-inflammatory effect. The results of western blotting and zymography showed the anti-invasion effect due to the decreased expression of MMP2 and MMP9 proteins in CRC cells after treatment with SCNE. Collectively, these data confirm the potential anticancer effects...
Embodiment 3
[0271] Example 3: CO of Neem (Azadirachta indica) 2 Leaf extract.
[0272] Take 1KG of clean and mature neem leaves and dry in shade to reduce moisture content to less than 12%. Make sure the powder contains less than 12% moisture. This dried herb is powdered to a particle size of less than 0.42mm and then heated between 80 bar (80kg / cm 2 ) and 350 bar (350kg / cm 2 ) at a pressure between 31°C and 45°C for SCO 2 extract. Depending on the size of the extractor and the amount of herbs loaded into the extractor at one time, the CO 2 Pass the herbs for a period of 2-3 hours. Depending on the solubility of lipophilic compounds present in the herb, the CO to be pumped through the herb 2 The amount in 10kg CO 2 / kg herbs to 40kg CO 2 Varies between / kg herbs. The CO2-carried extract is collected from the separator, where the CO 2 The pressure is reduced to a pressure that varies between 40 bar and 65 bar, and the temperature is between 10°C and 30°C, so that the solute (ext...
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