Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Compositions of azadirachta indica and methods of treating cancer

A composition and cancer technology, applied in the direction of drug combination, non-living material medical raw materials, drug delivery, etc., can solve the problems of unscalable industrial production, harmful changes in extraction temperature and separation temperature of plant components, and infeasibility of separation temperature

Pending Publication Date: 2021-06-25
BOARD OF RGT THE UNIV OF TEXAS SYST +1
View PDF8 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This large difference between extraction temperature and separation temperature can lead to unforeseen deleterious changes in plant components
Furthermore, the separation temperature is not commercially feasible or scalable for industrial production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Compositions of azadirachta indica and methods of treating cancer
  • Compositions of azadirachta indica and methods of treating cancer
  • Compositions of azadirachta indica and methods of treating cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0196] Example 1: Growth Inhibition of Oral Squamous Cell Carcinoma by Neem (Nem) Extract in Vivo: Tumor Reduction in Oral Squamous Cell Carcinoma by Disrupting the Inflammatory Cascade by Treatment with Natural Neem Leaf Extract occurrence and volume.

[0197]The leaves and bark of the neem tree (Neem) have been used for centuries in traditional Ayurvedic medicine to treat oral diseases. The experiments described herein tested the hypothesis that the initiation and / or progression of OSCC could be prevented using this neem leaf extract. The anticancer potential of neem leaf extract has been tested in in vitro and in vivo platforms. OSCC cell lines (SCC4, Cal27, HSC3) were treated with the leaf extract at different time points and markers of inflammation, invasion and proliferation were analyzed simultaneously. The preventive effect of SCNE was also evaluated in a heterotopic xenograft mouse model and a carcinogen-induced oral cancer mouse model. Treatment with neem leaf ext...

Embodiment 2

[0222] Example 2: Supercritical CO of Neem Leaves (Nem) 2 The extract and its bioactive limonoid, azadirachtin, inhibit colon cancer in preclinical models by modulating pro-inflammatory pathways.

[0223] To explore the role of neem in CRC, human colon cancer cell lines HCT116 and HT29 cells were treated with purified supercritical neem extract (SCNE) or azadirachtin. SCNE treatment showed dose-dependent inhibition of CRC cell proliferation and increased apoptosis. It was found that both SCNE and azadirachtin treatments showed decreased protein expression due to poor nuclear localization of p65, transcription factor phosphorylation STAT3, and pro-inflammatory cytokines COX1, COX2, IL-6, and TNF-α in CRC cells. Anti-inflammatory effect. The results of western blotting and zymography showed the anti-invasion effect due to the decreased expression of MMP2 and MMP9 proteins in CRC cells after treatment with SCNE. Collectively, these data confirm the potential anticancer effects...

Embodiment 3

[0271] Example 3: CO of Neem (Azadirachta indica) 2 Leaf extract.

[0272] Take 1KG of clean and mature neem leaves and dry in shade to reduce moisture content to less than 12%. Make sure the powder contains less than 12% moisture. This dried herb is powdered to a particle size of less than 0.42mm and then heated between 80 bar (80kg / cm 2 ) and 350 bar (350kg / cm 2 ) at a pressure between 31°C and 45°C for SCO 2 extract. Depending on the size of the extractor and the amount of herbs loaded into the extractor at one time, the CO 2 Pass the herbs for a period of 2-3 hours. Depending on the solubility of lipophilic compounds present in the herb, the CO to be pumped through the herb 2 The amount in 10kg CO 2 / kg herbs to 40kg CO 2 Varies between / kg herbs. The CO2-carried extract is collected from the separator, where the CO 2 The pressure is reduced to a pressure that varies between 40 bar and 65 bar, and the temperature is between 10°C and 30°C, so that the solute (ext...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
sizeaaaaaaaaaa
sizeaaaaaaaaaa
Login to View More

Abstract

The disclosure relates to compositions and methods of treating cancer in a subject. The method comprises administering to a patient in need of treatment an effective amount of supercritical CO2 extract of Azadirachta indica. The disclosure also relates to a process for preparing a CO2 extract of Azadirachta indica and herbal compositions thereof for the treatment of oral and colon cancers. The methods comprise a process for preparing a standardized SCO2 extract of Azadirachta indica leaves and herbal compositions of the same for oral use.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of the filing dates of U.S. Provisional Application No. 62 / 725,484, filed August 31, 2018, and Indian Application No. 201821021206, filed September 6, 2019. The contents of these earlier filed applications are hereby incorporated by reference in their entirety. technical field [0003] The present disclosure relates to the preparation of Neem (Azadirachta indica) CO for the treatment of oral cancer and colon cancer 2 Process of the extract and its herbal composition. More specifically, the present disclosure relates to a standardized SCO for the preparation of neem leaves for oral administration 2 Process of the extract and its herbal composition. Background technique [0004] As global smoking, alcohol consumption, and HPV exposure continue to rise, the risk of developing oral cancer is increasing worldwide. Conventional treatment improves the 5-year survival rate of patients wi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/31
CPCA61K36/58A61K2236/00A61K31/365A61K31/341A61K9/4858A61K9/4866A61K9/485A61K9/006A61K47/10A61K47/26A61K47/12A61K9/14A61K9/0053A61P35/00A61K2300/00A61K31/343A61P29/00A61K2236/37A61K31/355A61K9/4841A61K9/48A61K35/04A61K36/185A61K47/02A61K47/22A61K47/36A61K47/44A61K47/46
Inventor 吉里什·苏达卡·索曼M·沃戈维奇
Owner BOARD OF RGT THE UNIV OF TEXAS SYST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com