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Method and kit for simultaneously detecting vancomycin and norvancomycin in blood sample and application thereof

A technology for norvancomycin and blood samples, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of unstable signals, poor detection sensitivity, and low detection signals, and achieve rapid operation analysis, low cost, The effect of accurate test results

Inactive Publication Date: 2021-06-29
JINAN YING SHENG BIOTECH
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Problems solved by technology

[0005] Although there have been reports on the detection methods of vancomycin and norvancomycin in the prior art, the inventors have found that the existing pretreatment method to obtain the test solution usually has poor detection sensitivity, resulting in low sensitivity. Low detection signal on LC-MS, unstable signal

Method used

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  • Method and kit for simultaneously detecting vancomycin and norvancomycin in blood sample and application thereof
  • Method and kit for simultaneously detecting vancomycin and norvancomycin in blood sample and application thereof
  • Method and kit for simultaneously detecting vancomycin and norvancomycin in blood sample and application thereof

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Experimental program
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Embodiment

[0060] (1) Prepare the sample to be tested

[0061] Add the serum to the mixed solution of methanol and acetonitrile containing 0.05% zinc sulfate (methanol / acetonitrile volume ratio is 3 / 2, containing internal standard), shake and centrifuge, take out the supernatant after centrifugation, blow dry with nitrogen and flow The phase was redissolved to obtain the sample to be tested.

[0062] This embodiment compares zinc sulfate, formic acid, hydrochloric acid three kinds of additives, the concentration is respectively 0.05% zinc sulfate, 2% formic acid, 2% acetic acid, it is proved by the test that all can reduce the protein binding of vancomycin and norvancomycin The efficiency has obvious effect, and the concentration of each additive is optimized before the comparison experiment, and the best concentration of each is selected to participate in the comparison. The effect is shown in Table 1 below. Whether or not nitrogen blowing is obtained in the supernatant to be tested af...

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Abstract

The invention provides a method and a kit for simultaneously detecting vancomycin and norvancomycin in a blood sample and application of the method and the kit, and belongs to the technical field of medicine detection. The method for simultaneously detecting vancomycin and norvancomycin in the blood sample is successfully established by optimizing a sample pretreatment method and combining the sample pretreatment method with a high performance liquid chromatography-tandem mass spectrometry method, and has the advantages of simplicity in sample treatment, high sensitivity, high flux, reliable result and the like; the real-time monitoring on the blood level of the related antibiotics of the human body can be realized, so that the method has a good practical application value.

Description

technical field [0001] The invention belongs to the technical field of blood drug concentration monitoring, and in particular relates to a method for simultaneously detecting vancomycin and norvancomycin in a blood sample, a kit and an application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Vancomycin belongs to tricyclic glycopeptide antibacterial drugs, and it is mainly used clinically for endocarditis, osteomyelitis, For infectious diseases such as pneumonia, only 9 strains of Staphylococcus aureus in the world are drug-resistant, known as the last line of defense against antibiotics. Gram-positive (G + ) The problem of multi-drug resistance of bacteria ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/027G01N2030/045
Inventor 冯振景叶松弭兆元
Owner JINAN YING SHENG BIOTECH
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