Algae cell counting detection system and detection method thereof

A technology for algae cells and detection systems, which is applied to measuring devices, instruments, particle and sedimentation analysis, etc., can solve the problems of slow speed and time-consuming cell counting method, improve the effect and accuracy, increase the degree of recognition, and be more practical sexual effect

Active Publication Date: 2022-02-11
CHINA INST OF WATER RESOURCES & HYDROPOWER RES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cell counting method is slow, time-consuming, and generally needs to be operated in a laboratory, so it is not suitable for outdoor operations in an automated manner

Method used

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  • Algae cell counting detection system and detection method thereof
  • Algae cell counting detection system and detection method thereof
  • Algae cell counting detection system and detection method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] like figure 1 As shown, a kind of algae cell counting detection system comprises microscopic image acquisition system and image processing counting system, described microscopic image acquisition system is made up of digital camera A, microscope system B, flow cell C, flow pipeline D; The image processing and counting system is computer E, which includes image processing modules, data statistics and output modules;

[0033] The flow cell C is as figure 2 Shown: It is a box body 1 with an opening at one end. A liquid inlet pipe 2 and a liquid outlet pipe 3 are arranged on the two edges of the upper opening of the box body 1 respectively. The liquid inlet pipe 2 and the liquid outlet pipe 3 are displayed by a section of transparent cells. The tube 4 is connected, and the inner diameter of the cell display tube is 5-6 μm; the liquid inlet pipe and the liquid outlet pipe in the flow cell are made of stainless metal (such as stainless steel), and the inner diameter is 100 ...

Embodiment 2

[0041] This embodiment is a method for counting and detecting algae cells using the algae cell counting and detecting system in Example 1. Specific workflow such as Figure 4 shown.

[0042] The operation steps are:

[0043] 1) Turn on the solenoid valve, start the peristaltic pump, pump pure water to clean the pipeline for 3 minutes, and then turn off the power;

[0044] 2) Take the sample to be tested for 2 minutes, stop the peristaltic pump, and wait for 4 minutes to make the liquid in the flow cell static;

[0045] 3) Start the digital camera to take pictures, upload the picture data to the image processing and counting system to count algae cells, obtain the counting results, and save the pictures and counting results;

[0046] 4) Re-energize the solenoid valve and start the peristaltic pump to clean the pipeline;

[0047] 5) Steps 1) to 4) are repeated until the detection is completed, and the solenoid valve and the peristaltic pump are closed.

[0048] Through the ...

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Abstract

The invention discloses an algae cell counting detection system and a detection method thereof. The system includes a microscopic image acquisition system and an image processing counting system. The microscopic image acquisition system is composed of a digital camera, a microscope system, a flow pool, and a flow pipe; the image The processing and counting system is a computer, including image processing modules, data statistics and output modules. The algae cell counting detection system and the detection method thereof of the present invention can obtain algae density data conveniently, quickly and accurately, and provide data for early warning and control of algal blooms.

Description

technical field [0001] The invention relates to the technical field of detection of water-related environments, in particular to the detection of algae density, in particular to an algae cell counting detection system and a detection method thereof. Background technique [0002] Water bloom caused by eutrophication of water environment is becoming more and more serious in our country. Freshwater lakes such as Taihu Lake, Dianchi Lake, and Chaohu Lake experience cyanobacterial blooms every summer and autumn, and the dominant species is Microcystis that can produce microcystins. The occurrence of toxic cyanobacteria blooms not only brings serious harm to the water ecosystem, but also algae toxins have direct or potential harm to human health. Therefore, it is urgent to control the occurrence of algae blooms and eliminate the hazards of toxins. The monitoring of the number of Microcystis cells will provide first-hand information for early warning and control of algal blooms. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/14
CPCG01N15/1434G01N2015/1006
Inventor 张永生黄昱李海英封雷汤冬云崔洪亮
Owner CHINA INST OF WATER RESOURCES & HYDROPOWER RES
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