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Cooling device and cooling method used in stem cell cryopreservation process

A cooling device and stem cell technology, applied in household refrigeration devices, applications, household appliances, etc., can solve the problems of organelle damage, rupture, biological damage, etc., and achieve the effect of avoiding ice crystallization

Active Publication Date: 2022-05-20
挚量智控生物科技(山东)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] A cell with unlimited or immortal self-renewal ability, capable of producing at least one type of highly differentiated progeny cell stem cell is a stem cell, that is, a stem cell is a type of multipotential cell with self-replication ability. When preserving stem cells Low temperature is required for cryopreservation. The cryopreservation process will significantly change the thermodynamic, chemical and physical environment of the cells, and there is a danger of causing biological damage. When the stem cells are cryopreserved, large ice crystals are easily produced inside the cells, and the larger ice crystals will Cause damage to the cell membrane and some organelles in the cell and cause it to rupture. This is caused by the rapid drop in temperature when the cell is frozen. Ice crystallization occurs during cryopreservation. Therefore, we propose a cooling device and method for slow freezing to prevent crystallization in the process of stem cell cryopreservation

Method used

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  • Cooling device and cooling method used in stem cell cryopreservation process
  • Cooling device and cooling method used in stem cell cryopreservation process
  • Cooling device and cooling method used in stem cell cryopreservation process

Examples

Experimental program
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Effect test

Embodiment 1

[0052] A cooling method during the cryopreservation of stem cells:

[0053] S1: Replace the medium in the pre-prepared cell culture dish 24 hours before freezing, so that the cells are in the exponential growth phase;

[0054] S2: Collect the cultured cells by centrifugation, and then suspend the cells in the medium with serum added;

[0055] S3: Take the cryovial out of the liquid nitrogen and put it in a water bath at 42°C for 1 minute to melt the liquid;

[0056] S4: Take out the same amount of cryopreservation solution as the cell suspension, slowly add the cell suspension, and then shake to make a cell cryopreservation suspension;

[0057] S5: Put the prepared cryopreservation tubes on the rack 200, and then close the box 100;

[0058] S6: start the drive motor 401, the drive motor 401 rotates with the single-tooth gear 402, and the single-tooth gear 402 moves with the tooth plate 405 through the transmission gear 403 and the convex rod 404;

[0059] S7: The tooth plat...

Embodiment 2

[0061] A cooling method during the cryopreservation of stem cells:

[0062] S1: Replace the medium in the pre-prepared cell culture dish 36 hours before freezing, so that the cells are in the exponential growth phase;

[0063] S2: Collect the cultured cells by centrifugation, and then suspend the cells in the medium with serum added;

[0064] S3: Take the cryovial out of the liquid nitrogen and put it in a water bath at 42°C for 1.5 minutes to melt the liquid;

[0065] S4: Take out the same amount of cryopreservation solution as the cell suspension, slowly add the cell suspension, and then shake to make a cell cryopreservation suspension;

[0066] S5: Put the prepared cryopreservation tubes on the rack 200, and then close the box 100;

[0067] S6: start the drive motor 401, the drive motor 401 rotates with the single-tooth gear 402, and the single-tooth gear 402 moves with the tooth plate 405 through the transmission gear 403 and the convex rod 404;

[0068] S7: The toothed...

Embodiment 3

[0070] A cooling method during the cryopreservation of stem cells:

[0071] S1: Replace the medium in the pre-prepared cell culture dish 48 hours before freezing, so that the cells are in the exponential growth phase;

[0072] S2: Collect the cultured cells by centrifugation, and then suspend the cells in the medium with serum added;

[0073] S3: Take the cryovial out of the liquid nitrogen and put it in a water bath at 42°C for 2 minutes to melt the liquid;

[0074] S4: Take out the same amount of cryopreservation solution as the cell suspension, slowly add the cell suspension, and then shake to make a cell cryopreservation suspension;

[0075] S5: Put the prepared cryopreservation tubes on the rack 200, and then close the box 100;

[0076] S6: start the drive motor 401, the drive motor 401 rotates with the single-tooth gear 402, and the single-tooth gear 402 moves with the tooth plate 405 through the transmission gear 403 and the convex rod 404;

[0077] S7: The tooth pla...

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PUM

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Abstract

The invention relates to the technical field of cryopreservation of cells, and discloses a cooling device used in the process of cryopreservation of stem cells, which includes a box body, a placement frame, a refrigerator, a drive assembly and a slow flow assembly. The left side of the box is fixedly connected to the refrigerator, the right side of the inner wall of the refrigerator is fixedly connected to the slow flow assembly, and the drive assembly is fixedly connected to the top of the box. Cooling device and cooling method, the cone head block slowly enters the inner wall of the box and no longer blocks the flow rate in the intake pipe. The rod moves with the toothed plate, which makes the toothed plate rotate slowly, and the toothed plate rotates slowly with the connecting rod, so that the flow velocity inside the intake pipe increases slowly, the temperature inside the box decreases slowly, and large ice crystals appear inside the stem cells. .

Description

technical field [0001] The invention relates to the technical field of cell cryopreservation, in particular to a cooling device and a cooling method used in the process of stem cell cryopreservation. Background technique [0002] A cell with unlimited or immortal self-renewal ability, capable of producing at least one type of highly differentiated progeny cell stem cell is a stem cell, that is, a stem cell is a type of multipotential cell with self-replication ability. When preserving stem cells Low temperature is required for cryopreservation. The cryopreservation process will significantly change the thermodynamic, chemical and physical environment of the cells, and there is a danger of causing biological damage. When the stem cells are cryopreserved, large ice crystals are easily produced inside the cells, and the larger ice crystals will Cause damage to the cell membrane and some organelles in the cell and cause it to rupture. This is caused by the rapid drop in temperat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02F25D16/00
CPCA01N1/0221A01N1/0252F25D16/00
Inventor 房文彬
Owner 挚量智控生物科技(山东)有限公司