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Composite organic bacterial fertilizer as well as preparation method and application thereof

A technology of organic bacteria and bacteria liquid, applied in the field of compound fertilizer, can solve the problems of poor field colonization ability of effective active bacteria, few types of crops, and narrow adaptability of bacterial agent carriers.

Active Publication Date: 2021-07-16
GUANGDONG RICHHOLD BIOLOGICAL AGRI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the past, the effective and active strains of microbial agents were often blindly proportioned, lacked cooperation between strains, the carrier of the agent had a narrow adaptability to the climate, few types of crops, and poor tolerance to the diversity of the field environment. Bacteria have poor field colonization ability and effective bacteria have short potency

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  • Composite organic bacterial fertilizer as well as preparation method and application thereof
  • Composite organic bacterial fertilizer as well as preparation method and application thereof
  • Composite organic bacterial fertilizer as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The present embodiment provides a kind of preparation method of composite organic bacterial fertilizer, comprising the following steps:

[0044] (1) Single strain culture

[0045] Obtain single strains of nitrogen-fixing bacteria and hydrogenophilic bacteria through commercial channels, add them to the corresponding culture solution at a ratio of 1:1000 after resuscitating according to the regulations, put the inoculated culture solution into a constant temperature oscillator for cultivation, and adjust the temperature to 28°C. Rotate at 150rpm / min, culture for 24-48 hours, obtain nitrogen-fixing bacteria liquid and hydrogenophilic bacteria liquid respectively, insert the above-mentioned obtained nitrogen-fixing bacteria liquid and hydrogenophilic bacteria liquid into the plate culture medium for streaking culture, nitrogen fixation Bacteria streak plate and hydrogenophilic bacteria streak plate were as follows figure 1 and figure 2 shown.

[0046] Drop the cultivat...

Embodiment 2

[0061] In this embodiment, the performance test of the compound organic bacterial fertilizer prepared in Example 1 will be carried out through the following three aspects.

[0062] 1. Detection of nitrogen fixation ability of compound organic bacterial fertilizer

[0063] The co-cultivation fermentation liquid obtained in step (3) in Example 1, the nitrogen-fixing bacteria liquid obtained in step (1) were mixed with LB agar respectively to make a slant medium, and 5 mL of the slant medium was added to a test tube with a specification of 15mm*150mm At the same time, the same amount of clear water was used as a control, and the culture was sealed with a soft rubber stopper. After standing for 24 hours until the bacterial lawn grew, 5mL of air was pumped out, and 5mL of acetylene was injected. Ethylene production.

[0064] The nitrogen-fixing capacity of each bacterial agent is evaluated by the nmol amount of acetylene produced by each mg of bacterial liquid per hour. The higher...

Embodiment 3

[0076] In this example, the improvement effect of the compound organic bacterial fertilizer in Example 1 on soil will be explored through the following two aspects.

[0077] 1. The control effect of compound organic bacterial fertilizer on soil pH

[0078]Get Guangdong Qingyuan red soil, Changbai Mountain humus soil, fish pond pond bottom soil as test soil, every kind of soil sampling 10kg, add the co-cultivation fermentation liquid 100mL that embodiment 1 step (3) obtains, mix and stir, put into constant temperature and humidity culture The soil was cultured in a box, and samples were taken to detect the pH value of the soil on the 1st, 7th, and 28th days of cultivation.

[0079] The specific method of sampling and testing is as follows: take 100g of the test soil sample into a beaker, add distilled water to make it up to 1L, mix it with a vortex mixer and let it stand for 10min; take 100mL of the supernatant and pour it into a conical flask, and measure it with a pH meter. ...

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Abstract

The invention belongs to the technical field of compound fertilizers, and particularly relates to a compound organic bacterial fertilizer and a preparation method and application thereof. The method comprises the following steps: culturing single bacteria to respectively obtain nitrogen-fixing bacteria liquid and hydrogenophilic bacteria liquid; carrying out bacterial group co-culture on the nitrogen-fixing bacteria solution and the hydrogenophilic bacteria solution in a co-culture solution to obtain a primary co-culture bacteria solution; fermenting the primary co-culture bacterial liquid to obtain co-culture fermentation liquid; and infiltrating the co-culture fermentation liquor on a microbial agent carrier, and drying to obtain the composite organic bacterial fertilizer. On the basis of protection of the carrier on microorganisms and mutual symbiosis between nitrogen-fixing bacteria and hydrogenophils, the composite organic bacterial fertilizer has the advantages of being resistant to storage and transportation, capable of being colonized in plant rhizosphere for a long time, and suitable for various field environments.

Description

technical field [0001] The invention belongs to the technical field of compound fertilizers, and in particular relates to a compound organic bacterial fertilizer and its preparation method and application. Background technique [0002] In the past two centuries, with the vigorous development of chemical agriculture, the application of chemical fertilizers has increased year after year, and soil problems have become more and more serious. As a result, planters have to increase the amount of chemical fertilizers year after year to ensure or expand the original production, which in turn leads to soil problems. more serious. For the vast majority of our country's existing cultivated land, it is urgent to change the planting direction from the large-scale application of pesticides, chemical pesticides and hormones to an eco-friendly one that uses less or no pesticides and chemicals. In order to solve the problem of crop malnutrition under the existing arable land conditions, gro...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05G3/80C12N1/20C12R1/01C12R1/065
CPCC05F11/08C05G3/00C05G3/80C12N1/20Y02W30/40
Inventor 沈家葆李华一
Owner GUANGDONG RICHHOLD BIOLOGICAL AGRI CO LTD
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