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Polypeptide applied to inhibition of intracellular lipid accumulation and synthesis method thereof

A synthesis method and a cell-inhibiting technology are applied in the field of polypeptides that inhibit the accumulation of lipids in cells and their synthesis, which can solve the problems of patients failing to achieve dietary goals, threatening human life and health, and aggravating liver damage, and achieve alleviation and treatment of non-alcoholic Effects of fatty liver disease, alleviation of social and economic burden, and alleviation of accumulation

Active Publication Date: 2021-07-16
大连珍奥生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Non-alcoholic fatty liver disease is a chronic liver disease caused by metabolic syndrome. Its main feature is a large amount of fat accumulation in liver cells. Although the pathological process can be alleviated or even reversed by controlling diet and regular exercise in the early stage, most patients have not reached or maintained their dietary goals And weight loss, and with the continuous increase of lipid accumulation, liver damage is aggravated, non-alcoholic liver disease can develop into hepatitis, cirrhosis and even liver cancer, which greatly threatens human life and health

Method used

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  • Polypeptide applied to inhibition of intracellular lipid accumulation and synthesis method thereof
  • Polypeptide applied to inhibition of intracellular lipid accumulation and synthesis method thereof
  • Polypeptide applied to inhibition of intracellular lipid accumulation and synthesis method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A polypeptide used to inhibit lipid accumulation in cells and its synthesis:

[0040] 1) Put 1g of Fmoc-lys (boc) Wang resin into the reaction column, add 1.5g of DCM to soak for 5min, use 2g of DMF to wash and remove the DCM, and use 2g of 20% piperidine deprotectant to remove the Fmoc on the resin Protecting group, take off the cap for 20 minutes, then wash 6 times with DMF, if the color test is blue, then the Fmoc is successfully removed, if it is colorless, it means no reaction, and you need to take off the cap again;

[0041] 2) Take the second amino acid (arg) at the free carboxyl end, add 2g of condensing agent DIC and 2g of DMF into the above resin reaction column for reaction (gassing), then take the resin for ninhydrin color test reaction, the color result is a solution Bright yellow, transparent resin, no variegation;

[0042] 3) Use 2g of 20% piperidine deprotecting agent to remove the Fmoc protecting group on the second amino acid, wash with DMF for 6 time...

Embodiment 2

[0053] A polypeptide used to inhibit lipid accumulation in cells and its synthesis:

[0054] 1) Put 2g of Fmoc-lys (boc) Wang resin into the reaction column, add 3g of DCM to soak for 5min, use 4g of DMF to wash and remove the DCM, and use 4g of dioxane / 4M NaOH to remove the Fmoc on the resin Protecting group, take off the cap for 20 minutes, then wash 6 times with DMF, if the color test is blue, then the Fmoc has been successfully removed, if it is colorless, it means no reaction, and you need to take off the cap again;

[0055] 2) Take the second amino acid at the free carboxyl end, add condensing agent 3g DIC, 3g DMF and put it into the above resin reaction column for reaction (gassing), then take the resin for ninhydrin color test reaction, the color result is the solution is bright yellow, The resin is transparent, without variegation;

[0056] 3) Use 4g dioxane / 4M NaOH deprotecting agent to remove the Fmoc protecting group on the second amino acid, wash with DMF for 6 t...

Embodiment 3

[0061] A polypeptide used to inhibit lipid accumulation in cells and its synthesis:

[0062] 1) Put 1.5g of Fmoc-lys (boc) Wang resin into the reaction column, add 2.5g of DCM to soak for 5min, wash and remove the DCM with 4g of DMF, remove the Fmoc protecting group on the resin with 4g of pyrrolidone deprotecting agent, Take off the cap for 20 minutes, and then wash it with DMF for 6 times. If the color is blue, the Fmoc is successfully removed. If it is colorless, it means there is no reaction, and you need to take off the cap again;

[0063] 2) Take the second amino acid at the free carboxyl end, add condensing agent 5g DIC, 5g DMF and put it into the above resin reaction column for reaction (gassing), then take the resin for ninhydrin color test reaction, the color result is the solution is bright yellow, The resin is transparent, without variegation;

[0064] 3) Use 4g of pyrrolidone deprotecting agent to remove the Fmoc protecting group on the second amino acid, wash wi...

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Abstract

The invention belongs to the technical field of biological polypeptides, and particularly relates to a polypeptide applied to inhibition of intracellular lipid accumulation and a synthesis method thereof. The amino acid sequence of the polypeptide is as shown in a sequence table SEQ ID NO.1. The polypeptide has relatively high target affinity and high activity, and can show remarkable high activity under very low dosage and concentration, inhibit binding of lectin and sterol O-acyltransferase by exerting competition on the lectin and reduce the activity of the sterol O-acyltransferase, so as to achieve functions of controlling physiological metabolism in organisms, such as relieving lipid accumulation in hepatocytes, resisting tumor generation, assisting anti-cancer drugs in inhibiting tumor growth and the like. The synthetic process of the polypeptide is simple, the molecular weight of the polypeptide is small, the artificial synthesis efficiency is high, the difficulty of product purification in each step is greatly reduced through a solid-phase synthesis method, and the process is automatic, easy to control and low in cost.

Description

technical field [0001] The invention belongs to the technical field of biological polypeptides, and specifically relates to a polypeptide used for inhibiting intracellular lipid accumulation and a synthesis method thereof. Background technique [0002] With economic development, NAFLD has become the most common liver disease in the world. According to epidemiological statistics, patients with NAFLD account for about 25% of the global population. Especially in economically developed countries, such as the United States, about 30% to 40% of adults suffer from non-alcoholic fatty liver disease. In 2016, the annual economic loss caused by non-alcoholic fatty liver disease to the United States was about 103 billion US dollars. Non-alcoholic fatty liver disease is a chronic liver disease caused by metabolic syndrome. The main feature is a large amount of fat accumulation in liver cells. Although the pathological process can be alleviated or even reversed by controlling diet and re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/00C07K1/06C07K1/04A61K38/16A61P1/16A61P35/00
CPCC07K14/001A61P1/16A61P35/00A61K38/00Y02P20/55
Inventor 孙宏羽秦侬庄咏钧郑又玮
Owner 大连珍奥生物技术股份有限公司
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