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A polypeptide applied to inhibit intracellular lipid accumulation and its synthesis method

A synthesis method and lipid accumulation technology, applied in the preparation method of peptides, peptide/protein components, chemical instruments and methods, etc., can solve the problems of patients failing to meet dietary goals, threatening human life and health, and aggravating liver damage, etc., to achieve Relief and treatment of non-alcoholic fatty liver disease, relieve social and economic burden, relieve accumulation effect

Active Publication Date: 2022-01-11
孙宏羽
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Non-alcoholic fatty liver disease is a chronic liver disease caused by metabolic syndrome. Its main feature is a large amount of fat accumulation in liver cells. Although the pathological process can be alleviated or even reversed by controlling diet and regular exercise in the early stage, most patients have not reached or maintained their dietary goals And weight loss, and with the continuous increase of lipid accumulation, liver damage is aggravated, non-alcoholic liver disease can develop into hepatitis, cirrhosis and even liver cancer, which greatly threatens human life and health

Method used

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  • A polypeptide applied to inhibit intracellular lipid accumulation and its synthesis method
  • A polypeptide applied to inhibit intracellular lipid accumulation and its synthesis method
  • A polypeptide applied to inhibit intracellular lipid accumulation and its synthesis method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A polypeptide used to inhibit lipid accumulation in cells and its synthesis:

[0040] 1) Put 1g of Fmoc-lys (boc) Wang resin into the reaction column, add 1.5g of DCM to soak for 5min, use 2g of DMF to wash and remove the DCM, and use 2g of 20% piperidine deprotectant to remove the Fmoc on the resin Protecting group, take off the cap for 20 minutes, then wash 6 times with DMF, if the color test is blue, then the Fmoc is successfully removed, if it is colorless, it means no reaction, and you need to take off the cap again;

[0041] 2) Take the second amino acid (arg) at the free carboxyl end, add 2g of condensing agent DIC and 2g of DMF into the above resin reaction column for reaction (gassing), then take the resin for ninhydrin color test reaction, the color result is a solution Bright yellow, transparent resin, no variegation;

[0042] 3) Use 2g of 20% piperidine deprotecting agent to remove the Fmoc protecting group on the second amino acid, wash with DMF for 6 time...

Embodiment 2

[0053] A polypeptide used to inhibit lipid accumulation in cells and its synthesis:

[0054] 1) Put 2g of Fmoc-lys (boc) Wang resin into the reaction column, add 3g of DCM to soak for 5min, use 4g of DMF to wash and remove the DCM, and use 4g of dioxane / 4M NaOH to remove the Fmoc on the resin Protecting group, take off the cap for 20 minutes, then wash 6 times with DMF, if the color test is blue, then the Fmoc has been successfully removed, if it is colorless, it means no reaction, and you need to take off the cap again;

[0055] 2) Take the second amino acid at the free carboxyl end, add condensing agent 3g DIC, 3g DMF and put it into the above resin reaction column for reaction (gassing), then take the resin for ninhydrin color test reaction, the color result is the solution is bright yellow, The resin is transparent, without variegation;

[0056] 3) Use 4g dioxane / 4M NaOH deprotecting agent to remove the Fmoc protecting group on the second amino acid, wash with DMF for 6 t...

Embodiment 3

[0061] A polypeptide used to inhibit lipid accumulation in cells and its synthesis:

[0062] 1) Put 1.5g of Fmoc-lys (boc) Wang resin into the reaction column, add 2.5g of DCM to soak for 5min, wash and remove the DCM with 4g of DMF, remove the Fmoc protecting group on the resin with 4g of pyrrolidone deprotecting agent, Take off the cap for 20 minutes, and then wash it with DMF for 6 times. If the color is blue, the Fmoc is successfully removed. If it is colorless, it means there is no reaction, and you need to take off the cap again;

[0063] 2) Take the second amino acid at the free carboxyl end, add condensing agent 5g DIC, 5g DMF and put it into the above resin reaction column for reaction (gassing), then take the resin for ninhydrin color test reaction, the color result is the solution is bright yellow, The resin is transparent, without variegation;

[0064] 3) Use 4g of pyrrolidone deprotecting agent to remove the Fmoc protecting group on the second amino acid, wash wi...

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Abstract

The present invention is a technical field of biological polypeptides, and it involves a polypeptide and its synthetic methods for inhibiting the accumulation of lipids in the cell.The amino acid sequence of polypeptides is shown in the serial list SEQ ID No.1, which has high target affection and high activity. It can show significant high activity under a low dose and concentration. By exerting competition, competitionConcubationin, inhibitory condensate is combined with alcohol O -l alcohol metastases, reduces the activity of alcohol O -l alcohol metastases, and achieves the physiological metabolic function of the biological body, such as alleviating the accumulation of lipids in hepatocytes, fighting tumors, and assisting in anti -cancer anti -cancer.Drugs inhibit tumor growth; the synthesis process of polypeptides is simple, polypeptide molecular weight, and high efficiency of artificial synthesis. Through the solid phase synthesis method, the difficulty of the purification of each step is greatly reduced. The process is automated, easy to control, and low cost.

Description

technical field [0001] The invention belongs to the technical field of biological polypeptides, and specifically relates to a polypeptide used for inhibiting intracellular lipid accumulation and a synthesis method thereof. Background technique [0002] With economic development, NAFLD has become the most common liver disease in the world. According to epidemiological statistics, patients with NAFLD account for about 25% of the global population. Especially in economically developed countries, such as the United States, about 30% to 40% of adults suffer from non-alcoholic fatty liver disease. In 2016, the annual economic loss caused by non-alcoholic fatty liver disease to the United States was about 103 billion US dollars. Non-alcoholic fatty liver disease is a chronic liver disease caused by metabolic syndrome. The main feature is a large amount of fat accumulation in liver cells. Although the pathological process can be alleviated or even reversed by controlling diet and re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/00C07K1/06C07K1/04A61K38/16A61P1/16A61P35/00
CPCC07K14/001A61P1/16A61P35/00A61K38/00Y02P20/55
Inventor 孙宏羽秦侬庄咏钧郑又玮
Owner 孙宏羽
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