Method for shortening period of patient derived xenograft PDX model

Abstract

The invention discloses a method for shortening a period of a patient derived xenograft (PDX) model. The method comprises the following steps: pretreating tumor tissue of a patient or primary tumor cells derived from the tumor tissue by using bone marrow mesenchymal stem cells and matrigel during establishment of the PDX model; and preparing a preclinical tumor model by inoculating the treated tumor tissue of the patient or the primary tumor cells derived from the tumor tissue into an immunodeficient mouse body. The method can obviously shorten a construction period of a solid tumor PDX model and improve success rate, and is especially used for a preparation method for a highly invasive and metastatic cancer mouse model. The method has important value on early clinical evaluation, treatment and prognosis observation of solid tumors.

Description

technical field [0001] The invention relates to the medical field, and relates to a method for establishing an animal model, in particular to a method for establishing a human solid tumor tumor tissue for transplanting a tumor mouse model. Background technique [0002] One of the recognized shortcomings in the field of cancer research is the lack of model systems capable of studying carcinogenesis and cancer therapy. In order to evaluate the effect of cancer treatment in preclinical and clinical research, tumor animal models in which human tumor cell lines or patient-derived tumors are transplanted into immunodeficient mice are usually used, that is, human-derived tumor cell line xenografts (cell derived xenograft, CDX model) or human-derived tumor tissue-derived xenograft model (patientl derived xenograft, PDX model). Among them, the PDX model retains the primary tumor microenvironment and histopathological and genetic characteristics, which is of great significance for sc...

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Problems solved by technology

[0003] However, the PDX model cycle includes transplantation of human tumor cell lines or patient-derived tumors, proliferation and passage of tumor cells in mice, and the whole process takes about 6-12 months, which cannot meet the clinical needs of individualized chemotherapy regimens after surgery. need

Moreover, the success rate of PDX modeling is currently low, which is mainly limited by multiple factors such as tumor type, tumor malignancy, tumor cell ratio in tissue, tissue isolation time, recipient mice, operation technique, transplantation site, and feeding environment.

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