Method for determining content of triterpenoid saponin component in sapindus mukurossi

A technology for sapindus saponin and determination method, which is applied in the field of content determination of sapindus triterpenoid saponins, can solve problems such as surface activity, poor foaming property, uneven quality of raw materials, inability to effectively control quality, etc., and achieves short running time. , good resolution, high response value and high sensitivity

Pending Publication Date: 2021-07-30
株洲市食品药品检验所 +1
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  • Application Information

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Problems solved by technology

Or adopt high-performance liquid chromatography to measure the content of hedera saponin after hydrolyzing sapindus sapinsin, because hedera saponin has no surface activity, existing methods cannot accurately evaluate its quality
Experiments have shown that the surface activity and foaming properties of some purchased batches of sapindus saponin extract raw materials are not positively correlated with the sapindus saponin content indicated on the label, and some batches have very low saponin content measured by existing methods. high, but its surface activity and foaming property are very poor, suggesting that the raw materials of sapindus sapinsin extracts in the market are of uneven quality, and the existing methods cannot effectively control its quality

Method used

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  • Method for determining content of triterpenoid saponin component in sapindus mukurossi
  • Method for determining content of triterpenoid saponin component in sapindus mukurossi
  • Method for determining content of triterpenoid saponin component in sapindus mukurossi

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Determination method and results of Rarasaponin III, a triterpenoid saponin in Sapindus chinensis

[0042] 1.1. Drawing the standard curve: Accurately weigh an appropriate amount of Rarasaponin III reference substance, put it in a 10mL volumetric flask, dissolve it ultrasonically with 6mL methanol, constant volume, shake well, filter with a 0.45μm microporous membrane, take the subsequent filtrate, and dilute into a series of standards Working solutions: 69.80 μg / mL, 87.25 μg / mL, 174.5 μg / mL, 349.0 μg / mL, 698.0 μg / mL, 872.5 μg / mL. According to 1.3 chromatographic conditions sample injection analysis, obtain RarasaponinIII standard substance high performance liquid chromatogram (see figure 1 ); With the logarithm of peak area (A) as ordinate, the logarithm of concentration (C) as abscissa, carry out linear regression, regression equation is lnA=1.6937lnC-7.096(R 2=0.9984) (see Image 6 ).

[0043] 1.2. Preparation of the solution of the sample to be tested: Take the S...

Embodiment 2

[0049] Determination method and result of sapinmusaponion M content

[0050] 1.1. Drawing the standard curve: Accurately weigh about 10 mg of the Sapinmusaponion M reference substance, put it in a 10 mL volumetric flask, dissolve it with 6 mL of methanol sonication, constant volume, and shake well to make a solution containing 1000 μg per 1 mL, and use a 0.45 μm microporous filter membrane Filter, take the filtrate, and dilute into a series of standard working solutions: 50μg / mL, 62.5μg / mL, 125μg / mL, 250μg / mL, 500μg / mL, 625μg / mL. According to 1.3 chromatographic conditions sample injection analysis, obtain Sapinmusaponion M standard substance high performance liquid chromatogram (see figure 2 ); take the logarithm of peak area (A) as ordinate, and the logarithm of concentration (C) as abscissa, carry out linear regression, regression equation is lnA=1.6846lnC-6.3988(R 2 =0.9984) (see Figure 7 ).

[0051] 1.2. Preparation of the solution to be tested: same as step 1.2 of "...

Embodiment 3

[0055] Determination method and result of total saponin content of triterpenoid saponins in Sapindus chinensis

[0056] 1.1. Standard curve drawing: Accurately weigh an appropriate amount of triterpenoid total saponins reference substance, put it in a 10mL volumetric flask, dissolve it with 6mL of methanol sonication, constant volume, shake well, filter with a 0.45μm microporous membrane, take the subsequent filtrate, and dilute A series of standard working solutions: 1.023mg / mL, 2.047mg / mL, 4.094mg / mL, 8.187mg / mL, 10.23mg / mL. According to 1.3 chromatographic conditions sample introduction analysis, obtain total saponin standard substance high performance liquid chromatography (see image 3 ); With the logarithm of peak area (A) as ordinate, the logarithm of concentration (C) as abscissa, carry out linear regression, regression equation is lnA=1.6271lnC+3.2131(R 2 =0.9983) (see Figure 8 ).

[0057] 1.2. Preparation of the solution to be tested: same as step 1.2 of "Example...

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Abstract

The invention relates to a method for determining the content of a triterpenoid saponin component in sapindus mukurossi, which comprises the following steps of: (1) obtaining a chromatographic standard curve of a sapindoside characteristic component according to a series of concentration standard solutions of the sapindoside characteristic component, and establishing a standard curve equation; (2) preparing a to-be-detected sample solution; (3) based on the solution prepared in the step (2), detecting the to-be-detected solution by adopting a high performance liquid chromatography-evaporative light scattering method; and (4) calculating the concentration of the sapindoside characteristic component in the to-be-detected sample according to the chromatographic peak area detected in the step (3) and the standard curve equation established in the step (1), converting the concentration of the sapindoside characteristic component in the to-be-detected sample into the content of a saponin characteristic component in the to-be-detected sample, and judging the quality of the to-be-detected sample accordingly. The method is short in operation time, high in response value and sensitivity, good in separation degree between peaks, capable of accurately measuring the content of the sapindus saponin, and suitable for quality control of the sapindus pericarp and the sapindus saponin extract raw materials.

Description

technical field [0001] The invention relates to a method for determining the content of triterpenoid saponins in sapindus chinensis, belonging to the technical field of biological detection. Background technique [0002] Sapindaceae (Sapindaceae), commonly known as "linden tree, soap fruit, soap tree", etc., has a Latin name of Sapindus, which means "Indian soap". "Compendium of Materia Medica" records that the fruit of Sapindus chinensis can be used to wash hair and face, because the peel is rich in saponins, most of which are triterpenoid saponins, that is, Sapindus sapindosides, with a total content of about 5.33%. Sapindus saponin is a natural non-ionic surfactant with low critical micelle concentration and surface tension, which can significantly reduce the surface tension of water, rich foam, delicate hand feeling, strong detergency, 100% degradation, long-term The use will not be harmful to the human body and the environment, and it is more and more widely used in th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/74G01N30/86
CPCG01N30/02G01N30/74G01N30/8631
Inventor 吴泽君陈珉珉李洪权唐小鹏董华贵吴茜符春花彭怡心
Owner 株洲市食品药品检验所
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