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Pongamia compositions, methods of preparing and analyzing thereof, and uses thereof

A technology of pongamia and composition, which is applied in the field of pumella oilseed products, can solve the problems of inaccuracy and failure to provide internally consistent reference scales, etc.

Pending Publication Date: 2021-07-30
TERVIVA BIOENERGY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since these methods rely on the efficiency of methanol or hexane extraction, and this efficiency will vary for each method depending on the nature of the material being analyzed and its previous processing history, these methods report Inaccurate values ​​of pangetin and other chemical compounds in
Also, different analytical methods reported different oil, pangetin, and pangetin concentrations for the same pongamia seed cake sample, and often did not provide an internally consistent reference scale across different treatments
Therefore, it is difficult in the field to make meaningful comparisons of different treatments based on existing analytical methods

Method used

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  • Pongamia compositions, methods of preparing and analyzing thereof, and uses thereof
  • Pongamia compositions, methods of preparing and analyzing thereof, and uses thereof
  • Pongamia compositions, methods of preparing and analyzing thereof, and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment A

[0222] Example A: Analytical method

Embodiment A1

[0223] Example A1 : Comparing Methods of Methanol-Based Extraction

[0224] The following examples describe experiments comparing the extraction of pangetin and pangetin from pumice seed cake using methanol as a solvent.

[0225] Homogeneous Extraction of Pantanthin and Pantanthin. Place 0.5 g of Pungacia seed cake with 5 mL of methanol in a final ratio of 10:1 (solvent:solid) in a 50 mL polypropylene centrifuge tube. Then, the samples were placed in a plant / tissue homogenizer and shaken at 1500 rpm for 2 minutes. Next, the samples were centrifuged at 3000 rpm for 5 minutes to separate the solvent from the solids, and the supernatant was slowly poured into a clean 50 mL polypropylene tube. The extraction process was repeated 5 times to improve the extraction of tangeretin and tangeretin.

[0226] Homogeneous extraction with NaOH immersion. Before the homogeneous extraction, 0.5 g of Pseudomonas seed cake was placed in a 50 mL polypropylene centrifuge tube with 1 mL of 2% N...

Embodiment A2

[0251] Example A2: Solvent dependence of the extraction method

[0252] The following examples describe the experimental work that evaluated the ability of various solvents to extract pangetin and pongitin from pongamia seed cake.

[0253] Homogeneous Extraction of Pantanthin and Pantanthin. In this example, homogeneous extraction was performed as in Example A1. However, the solvents tested included ethanol, hexane, methyl tert-butyl ether (MTBE), toluene, diethyl ether, ethyl acetate, and acetone, all at a 50:1 solvent:solid ratio. Finally, an experiment was performed to evaluate the effect of homogenization time on ethyl acetate solvent extraction in which 10-minute (2 min / cycle, 5 cycles) and 50-minute (10 min / cycle, 5 cycles) homogenizations were compared.

[0254] Soxhlet extraction of pianitin and pianitin. In this example, Soxhlet extraction was performed as in Example A1. However, the solvents tested were methanol, MTBE and ethyl acetate. Further, the reaction tim...

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Abstract

The present disclosure relates to pongamia compositions having low concentrations of karanjin and other active chemical components intrinsic to pongamia oilseeds, methods of preparing and using said pongamia compositions. The present disclosure also relates to methods of analyzing pongamia compositions, as well as uses of the pongamia compositions.

Description

[0001] Cross References to Related Applications [0002] This application claims priority and benefit to U.S. Provisional Application No. 62 / 741,351, filed October 4, 2018, the entire disclosure of which is incorporated herein by reference in its entirety. technical field [0003] The present disclosure relates generally to pongamia oilseed products, and more particularly to pongamia compositions having low concentrations of residual pongamia oil, pongamia, and pongamia seeds, and to making and Ways to use this Psakami composition. The present disclosure also relates to methods of analyzing Pseudomonas compositions prepared by the methods described herein, as well as other treatments known in the art. The present disclosure also relates to the use of the pongamica composition as feed for cattle and other ruminants. Background technique [0004] Pongana seed cake, a by-product of extracting oil from pongana oleaginous seeds, offers a potentially renewable source of protein ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/14A23K10/30A23K10/37
CPCA23K10/37A23J1/14A23J1/142A23J1/146A23K50/10A23K20/10Y02P60/87A23K10/30B01D11/0288B01J19/126
Inventor N·西卡W·N·库施V·K·拉尼
Owner TERVIVA BIOENERGY INC