Pre-filled culture dish containing beta-lactamase and preparation method thereof

Abstract

The invention belongs to the technical field of culture dish filling and particularly relates to a pre-filled culture dish containing a beta-lactamase and a preparation method thereof. The pre-filled culture dish comprises a culture medium storage tank, a beta-lactamase storage tank, a stirring tank A and a stirring tank B; the culture medium storage tank communicates and is connected with a first three-way pipe, a first tank bottom valve, a second tank bottom valve, a second discharging pipe, a first discharging pipe, a first three-way valve, a first feeding pipe, a first circulating pipe, a second three-way valve, a second feeding pipe and a second circulating pipe; and the beta-lactamase storage tank communicates and is connected with a second three-way pipe, a third tank bottom valve, a fourth tank bottom valve, a fourth discharging pipe, a third discharging pipe, a third three-way valve, a third feeding pipe, a third circulating pipe, a fourth three-way valve, a fourth feeding pipe and a fourth circulating pipe, and the stirring tank A and the stirring tank B are connected with a filling mechanism through a fifth tank bottom valve, a sixth tank bottom valve and a fifth three-way valve. A culture medium and a beta-lactamase solution can be stored, fluidity of the culture medium and activity of the beta-lactamase are ensured by controlling states of the valves, such that the quality control level of the pre-filled culture dish containing beta-lactamase is effectively improved.

Description

technical field [0001] The invention belongs to the technical field of culture dish filling, and in particular relates to a pre-filled culture dish with penicillinase and a preparation method thereof. Background technique [0002] Penicillinase, also known as β-lactamase (β-lactamase, EC 3.5.2.6), can hydrolyze β-lactam antibiotics. It is widely distributed in various microorganisms, especially in bacteria. Penicillinase crystals with molecular weights of 35,200 and 31,500 were obtained from Bacillus cereus5 / B and B. cereus 569, respectively. α, β, and γ penicillinase crystals were extracted from B. megaterium, and then isolated from different bacteria A variety of penicillinases. Penicillinase is extremely unstable to heat, and the speed of decomposing β-lactam varies with the type of penicillin and enzyme, but it has a weak effect on 6-aminopenicillanic acid (6-APA). The optimum pH value of the enzyme reaction is 6.0-6.5. With penicillin G potassium salt as the substrat...

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Problems solved by technology

In the process of making the medium containing penicillinase, in order to ensure that the penicillinase can be evenly mixed in the medium, it is generally selected to use the penicillinase solution as a raw material to add to the flowing medium; however, after adding the penicillinase to the melting When in the agar medium in the state, because the temperature of the medium is much higher than the temperature of 2-8°C suitable for storing the penicillinase solution, if the medium cannot be cooled and solidified in a short time, it will be harmful to the penicillinase. activity has a greater impact on

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