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Culture medium and culture method for inducing differentiation of iPS (induced pluripotent stem) cells

A cell differentiation and cell culture technology, applied in the direction of cell culture active agents, artificially induced pluripotent cells, biochemical equipment and methods, etc., can solve problems such as hindering the application of induced differentiated cardiomyocytes, and achieve high repeatability and high purity. , the effect of stable culture conditions

Pending Publication Date: 2021-08-10
华夏源细胞工程集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The existing induced differentiation cardiomyocytes contain animal-derived components, which involves ethical issues, which makes the inclusion of animal-derived components hinder the late clinical application of induced differentiation cardiomyocytes

Method used

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  • Culture medium and culture method for inducing differentiation of iPS (induced pluripotent stem) cells
  • Culture medium and culture method for inducing differentiation of iPS (induced pluripotent stem) cells
  • Culture medium and culture method for inducing differentiation of iPS (induced pluripotent stem) cells

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Embodiment 1

[0031] An embodiment of the present invention provides a medium for inducing iPS cell differentiation. The medium for inducing iPS cell differentiation includes: the first medium for myocardial differentiation, the second medium for myocardial differentiation, and the basal medium for myocardial differentiation. The first medium for myocardial differentiation is The base includes: the first solute and the first solvent, the first solute includes: human serum albumin, ascorbic acid and glycogen synthase kinase inhibitor CHIR99021, the first solvent includes: RPMI-1640; the second medium for myocardial differentiation includes: the second The second solute and the second solvent, the second solute includes: human serum albumin, ascorbic acid and inhibitor IWP-2, the second solvent includes: RPMI-1640, the myocardial differentiation basal medium includes the third solute and the third solvent, the third The solutes include: human serum albumin and ascorbic acid, and the third solv...

Embodiment 2

[0051] In one aspect, the present invention provides a culture medium for inducing iPS cell differentiation. The culture medium for inducing iPS cell differentiation includes: a first medium for myocardial differentiation, a second medium for myocardial differentiation and a basic medium for myocardial differentiation. The first medium for myocardial differentiation is The medium includes: the first solute and the first solvent, the first solute includes: human serum albumin, ascorbic acid and glycogen synthase kinase inhibitor CHIR99021, the first solvent includes: RPMI-1640; the second medium for myocardial differentiation includes: The second solute and the second solvent, the second solute includes: human serum albumin, ascorbic acid and inhibitor IWP-2, the second solvent includes: RPMI-1640, the myocardial differentiation basal medium includes the third solute and the third solvent, the second The three solutes include: human albumin and ascorbic acid, and the third solve...

Embodiment 3

[0070] An embodiment of the present invention provides a medium for inducing iPS cell differentiation. The medium for inducing iPS cell differentiation includes: the first medium for myocardial differentiation, the second medium for myocardial differentiation, and the basal medium for myocardial differentiation. The first medium for myocardial differentiation is The base includes: the first solute and the first solvent, the first solute includes: human serum albumin, ascorbic acid and glycogen synthase kinase inhibitor CHIR99021, the first solvent includes: RPMI-1640; the second medium for myocardial differentiation includes: the second The second solute and the second solvent, the second solute includes: human serum albumin, ascorbic acid and inhibitor IWP-2, the second solvent includes: RPMI-1640, the myocardial differentiation basal medium includes the third solute and the third solvent, the third The solutes include: human serum albumin and ascorbic acid, and the third solv...

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Abstract

The invention discloses a culture medium and a culture method for inducing iPS cell differentiation. The culture medium for inducing differentiation of the iPS cells comprises a myocardial differentiation first culture medium, a myocardial differentiation second culture medium and a myocardial differentiation basic culture medium; the myocardial differentiation first culture medium comprises a first solute and a first solvent, the first solute comprises human serum albumin, ascorbic acid and a glycogen synthase kinase inhibitor CHIR99021, and the first solvent comprises RPMI-1640; the myocardial differentiation first culture medium comprises a first solute and a first solvent, the myocardial differentiation second culture medium comprises a second solute and a second solvent, the second solute comprises human albumin, ascorbic acid and an inhibitor IWP-2, the second solvent comprises RPMI-1640, the myocardial differentiation basic culture medium comprises a third solute and a third solvent, the third solute comprises human albumin and ascorbic acid, and the third solvent comprises RPMI-1640. The culture medium for inducing the differentiation of the iPS cells does not contain other species source components.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a culture medium and a culture method for inducing iPS cell differentiation. Background technique [0002] iPS cells (Induced pluripotent stem cells, induced pluripotent stem cells) are stem cells induced from somatic cells, which have developmental pluripotency similar to embryonic stem cells. iPS cells can be induced to differentiate into cardiomyocytes and have potential applications in the regenerative therapy of heart disease. [0003] The existing induced differentiation cardiomyocytes contain animal-derived components, which involves ethical issues, which makes the animal-derived components hinder the later clinical application of induced differentiation cardiomyocytes. Contents of the invention [0004] In order to solve the problems of the prior art, the embodiment of the present invention provides a culture medium and a culture method for inducing differentiation of iPS ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077
CPCC12N5/0657C12N2506/45C12N2500/38C12N2501/727C12N2501/415
Inventor 李璐佳
Owner 华夏源细胞工程集团股份有限公司
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