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Method for detecting cystic echinococcosis

A technology for cystic echinococcosis and subjects, applied in the field of medical testing, can solve the problems of standardization, missed detection, lack of specificity and sensitivity that are difficult to detect, and achieve the effect of improving the accuracy rate

Pending Publication Date: 2021-08-10
中国人民解放军陆军第九五八医院
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Problems solved by technology

However, the coating antigen used in the current commercial kits is generally extracted from the cyst fluid of the infected part of Echinococcus, and it is difficult to standardize the detection
This method lacks high specificity and sensitivity, and also increases the possibility of false positive results (such as cross-reactions with other parasites), and in actual testing, missed and wrong results often occur

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  • Method for detecting cystic echinococcosis
  • Method for detecting cystic echinococcosis

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Embodiment Construction

[0023] The following will clearly and completely describe the technical solutions in the embodiments of the application with reference to the drawings in the embodiments of the application. Apparently, the described embodiments are only some of the embodiments of the application, not all of them. Based on the embodiments in this application, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of this application.

[0024] The terms "first", "second" and the like in the specification and claims of the present application and the above drawings are used to distinguish different objects, rather than to describe a specific order. Furthermore, the terms "include" and "have", as well as any variations thereof, are intended to cover a non-exclusive inclusion. For example, a process, method, system, product or device comprising a series of steps or units is not limited to the listed steps or units, bu...

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Abstract

The embodiment of the invention provides a method for detecting cystic echinococcosis. The method comprises the following steps: coating an ELISA plate with an Ag5 antigen which is recombined and induced by a prokaryotic expression system and is purified in vitro; diluting serum of a subject in proportion, and adding the diluted serum and known negative and positive serum into the plate holes of the ELISAplate for reaction; and measuring the OD450 value of each plate hole, and calculating the sample value of each plate hole according to the publicity. Clinical serum sample detection shows that the detection specificity and sensitivity are higher than those of a commercial kit, and false positive results are effectively eliminated. The indirect ELISA method established in the invention provides a more accurate and effective detection means for serological diagnosis of echinococcosis.

Description

technical field [0001] The application relates to the technical field of medical detection, in particular to a method for detecting cystic echinococcosis. Background technique [0002] Cystic echinococcosis (CE), also known as cystic echinococcosis, is a zoonotic parasitic disease caused by the larvae of Echinococcus granulosus (Eg). After the patient is infected, there will be an asymptomatic incubation period of several years. Due to the spread and localization of the cyst, different types of symptoms will appear, such as: pain, jaundice, vomiting of bile, chest pain and mild paralysis. The latest epidemiological research shows that at least 270 million people (accounting for 58% of the total population) in Central Asia are infected with echinococcosis, which can cause direct or indirect economic losses of 194 million US dollars every year. It is conservatively estimated that the global minimum disability-adjusted life expectancy (HALE) due to echinococcosis is about 28.5...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/543
CPCG01N33/543G01N33/68G01N2333/43543
Inventor 余闻静龙宇鹏
Owner 中国人民解放军陆军第九五八医院
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