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A kind of Streptococcus thermophilus stn26, bacterial powder and application in uric acid lowering product

A Streptococcus thermophilus, STN26 technology, applied in the field of microorganisms, can solve the problems of poor microbial viability, influence on microbial colonization, poor stability of curative effect, etc., and achieve the effect of convenient taking, good adaptability and customization ability, and good activity.

Active Publication Date: 2021-10-01
中科嘉亿营养医学(山东)微生态研究院有限公司
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Problems solved by technology

Among them, diet and exercise are the healthiest method, but the effect of this method is poor, and it is difficult to adhere to
Mechanisms of drug treatment methods are divided into two types. One is to promote the excretion of uric acid by the kidneys. The commonly used western medicine is benzbromarone, and the traditional Chinese medicines are Smilax, Siwu Decoction and Ermiaosan. Reabsorption to reduce uric acid in the blood; the second is to inhibit the metabolism of purine in the liver and small intestine to produce uric acid. The commonly used western medicine is allopurinol, and the traditional Chinese medicine is Jianpi Huashi Decoction and Tongfeng Granules. The above-mentioned medicine is a xanthine oxidase inhibitor , by combining with xanthine oxidase to inhibit the oxidation of hypoxanthine to form uric acid and reduce the entry of uric acid into the blood; , will produce side effects such as allergies and in vivo toxicity, especially western medicine, which has a more serious impact on the human body when taken for a long time; although traditional Chinese medicine has small side effects, its curative effect is less stable
Microbial therapy is a relatively safe treatment method. Its mechanism is to regulate the balance of intestinal flora, promote the decomposition of intestinal uric acid by microorganisms, and then achieve the effect of reducing uric acid in the blood; most of the commonly used microbial preparations are oral preparations, oral preparations It needs to pass through the stomach before entering the intestinal tract. Because the pH value in the gastric juice is too low, the survival of microorganisms is poor, which affects the colonization of microorganisms in the intestinal tract, thereby affecting the curative effect

Method used

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  • A kind of Streptococcus thermophilus stn26, bacterial powder and application in uric acid lowering product
  • A kind of Streptococcus thermophilus stn26, bacterial powder and application in uric acid lowering product
  • A kind of Streptococcus thermophilus stn26, bacterial powder and application in uric acid lowering product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation and Identification of Bacterial Strains

[0029] 1. Strain screening and purification

[0030] (1) Sample preparation:

[0031] ①Put sterilized saline (0.85%) into a sterile triangular flask, then add 1ml of naturally fermented yoghurt from a herdsman's home in Yili, Xinjiang, to it, shake, and set aside;

[0032] ② Carry out 10 times gradient dilution to the solution of step ①, respectively 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 ,stand-by;

[0033] (2) Prepare medium:

[0034] The composition of the MRS plate medium is as follows:

[0035] Peptone 10g, beef powder 5g, sodium acetate trihydrate 5g, dipotassium hydrogen phosphate heptahydrate 2g, Tween 80 1mL, manganese sulfate tetrahydrate 0.05g, triammonium citrate 2g, glucose 20g, magnesium sulfate heptahydrate 0.2g, agar 15g, distilled water 1000mL; mix the above raw materials, adjust the natural pH, stir the bacterial liquid, and sterilize at 121°C, 0.1MPa for 20min; ;

[00...

Embodiment 2

[0048] Embodiment 2 preparation product

[0049] (1) Preparation of MRS liquid medium:

[0050] The composition of MRS liquid medium is peptone 10g, beef powder 5g, sodium acetate trihydrate 5g, dipotassium hydrogen phosphate heptahydrate 2g, Tween 80 1mL, manganese sulfate tetrahydrate 0.05g, triammonium citrate 2g, glucose 20g, seven Magnesium sulfate water 0.2g, distilled water 1000mL; after mixing the above raw materials, adjust the pH to 6.8, stir the bacteria solution, and sterilize at 121°C and 0.1MPa for 20min.

[0051] (2) Preparation of bacteria powder:

[0052] The Streptococcus thermophilus STN26 preserved in Example 1 was inoculated on the MRS plate medium, activated and cultured at 37°C for 48h, then inoculated in the MRS liquid medium according to the inoculum size of 1%, and cultivated at 37°C for 24h , to obtain the bacterial liquid;

[0053] After the bacterial liquid is centrifuged, the bacterial cells are collected, washed with sterile physiological sali...

Embodiment 3

[0056] Example 3 Streptococcus thermophilus STN26 passed the simulated gastrointestinal digestive juice experiment

[0057] 1. Preparation and operation of simulated gastric juice

[0058] (1) The simulated gastric juice was prepared according to the following method: dilute with 9.5% hydrochloric acid and distilled water to pH 1.5, add 1.0 g of pepsin per 100 mL, mix well, filter with a 0.22 μm sterile filter membrane, and prepare and use immediately.

[0059] (2) Experimental process:

[0060] ①Weigh 1g of the product of specification B, as a sample, for use;

[0061] ②Take 9mL of simulated gastric juice from step (1) in a test tube, preheat it to 37°C, then put the sample in step ① into the test tube, and place it at 37°C and 80r / min for 1h, 2h, 3h and 4h respectively. Each treatment was repeated 3 times;

[0062] ③ Detection: Gradiently dilute the treated sample solution, use the pouring method to count viable bacteria, and then calculate the survival rate. The results ...

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Abstract

The invention provides a Streptococcus thermophilus STN26, bacteria powder and its application in uric acid-lowering products. The Streptococcus thermophilus STN26 was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on May 27, 2021, referred to as CGMCC, Address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, No.: CGMCC No.22618, Classification Name: Streptococcus thermophilus Streptococcus thermophilus . When the product prepared by using the Streptococcus thermophilus STN26 is applied, it can effectively reduce the uric acid level and relieve the symptoms of gout, and the effect is remarkable.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a streptococcus thermophilus STN26, bacterial powder and an application in uric acid-lowering products. Background technique [0002] Uric acid is a product of purine metabolism, which is easy to form crystals; when purine metabolism is disordered, uric acid will precipitate in the joints along with blood flow in the human body, causing gout. [0003] Common treatments for high uric acid include diet, exercise, drug therapy, and microbial therapy. Among them, diet and exercise are the healthiest method, but the effect of this method is relatively poor, and it is difficult to persist. Mechanisms of drug treatment methods are divided into two types. One is to promote the excretion of uric acid by the kidneys. The commonly used western medicine is benzbromarone, and the traditional Chinese medicines are Smilax, Siwu Decoction and Ermiaosan. Reabsorption to reduce uric acid ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/744A61K47/26A61P19/06C12R1/46
CPCA61K35/744A61K47/26A61P19/06C12N1/20
Inventor 侯建亮张永娟潘玉林曹维超李翠华潘仕城刘红亮司书锋孙川周文浩步敏
Owner 中科嘉亿营养医学(山东)微生态研究院有限公司
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