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A blood cell microfluidic separation device and method combining magnetophoresis and dielectrophoresis

A separation device and dielectrophoresis technology, applied in the field of cell separation, can solve the problems of difficulty in detection and analysis

Active Publication Date: 2022-06-24
DALIAN MARITIME UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Detection and analysis of these cells is difficult due to the low concentration of these cells in the blood

Method used

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  • A blood cell microfluidic separation device and method combining magnetophoresis and dielectrophoresis
  • A blood cell microfluidic separation device and method combining magnetophoresis and dielectrophoresis

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Embodiment

[0055]The sheath fluid and the blood sample are introduced from the first liquid inlet 100 and the second liquid inlet 101 respectively, and the blood sample will be squeezed by the sheath liquid with a higher flow rate to the side of the main channel 10 close to the ferromagnet 2. At this time, the blood The sample enters the magnetophoretic separation zone. The leukocytes and circulating tumor cells subjected to the strong negative magnetophoretic force in this area are repelled by the ferromagnet 2 and flow into the first secondary channel 11 , while the red blood cells subjected to the positive magnetophoresis force and the platelets subjected to the weaker negative magnetophoresis force flow into the second secondary channel 12. The cells flowing into the first secondary channel 11 and the second secondary channel 12 will enter the dielectrophoretic separation area. By adjusting the AC electric field and the asymmetric pore structure, the cells were separated and flowed ...

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Abstract

The invention provides a blood cell microfluidic separation device and method combining magnetophoresis and dielectrophoresis. The device of the invention includes: a microchannel, two liquid inlets, four liquid outlets, a permanent magnet, a ferromagnet, two pairs of electrode. According to the flow direction of the sample, the device is provided with a magnetophoretic separation area and a dielectrophoretic separation area in sequence. The magnetophoretic separation area uses a permanent magnet to generate a magnetic field to separate the mixed sample of red blood cells and platelets from the mixed sample of circulating tumor cells and white blood cells. The dielectrophoretic separation area utilizes an asymmetric pore structure to generate a high-gradient non-uniform electric field to separate red blood cells from platelets and circulating tumor cells from white blood cells. The present invention uses magnetophoresis as the pre-enrichment stage of dielectrophoresis separation to realize primary separation, and utilizes dielectrophoresis to realize secondary separation, thereby realizing the continuous separation of circulating tumor cells, red blood cells, platelets and white blood cells in blood samples without pre-labeling cells Sample, does not affect cell physiological activity.

Description

technical field [0001] The present invention relates to the technical field of cell separation, in particular, to a blood cell microfluidic separation device and method combining magnetophoresis and dielectrophoresis. Background technique [0002] Isolation of cells is an essential step in cell biology research and many diagnostic and therapeutic methods. Because blood is an extremely informative and easily accessible tissue, many current diagnostic tests rely on isolated blood components, such as white blood cells, which are required for several hematology tests and DNA sequencing. Blood components are also used therapeutically, and transfusions of purified platelets are often required during surgery. Some special cells in the blood often indicate a certain disease, such as monocytes, which are markers of diseases related to abnormal red blood cell turnover. Another example is circulating tumor cells, which are rare cells that can be found in patients before the primary t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00
CPCB01L3/502753B01L3/50273B01L2400/0424B01L2400/043
Inventor 王俊生赵鹏璐陈斌赵迅赵凯
Owner DALIAN MARITIME UNIVERSITY
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