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Blood cell microfluidic separation device and method combining magnetophoresis and dielectrophoresis

A separation device, a technology for blood cells, applied in the field of cell separation, which can solve problems such as difficulty in detection and analysis

Active Publication Date: 2021-08-17
DALIAN MARITIME UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Detection and analysis of these cells is difficult due to the low concentration of these cells in the blood

Method used

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  • Blood cell microfluidic separation device and method combining magnetophoresis and dielectrophoresis
  • Blood cell microfluidic separation device and method combining magnetophoresis and dielectrophoresis

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Embodiment

[0055]The sheath liquid and the blood sample are respectively introduced from the first liquid inlet 100 and the second liquid inlet 101, and the blood sample will be squeezed to the side of the main channel 10 close to the ferromagnet 2 by the sheath liquid with a higher flow rate. The sample enters the magnetophoretic separation area. Leukocytes and circulating tumor cells subjected to stronger negative magnetophoretic force in this area are repelled by ferromagnet 2 and flow into the first secondary channel 11, while red blood cells subjected to positive magnetophoretic force and platelets subjected to weaker negative magnetophoretic force flow into the second secondary channel 12. Cells flowing into the first secondary channel 11 and the second secondary channel 12 will enter the dielectrophoretic separation area. By adjusting the AC electric field and the asymmetric pore structure, the cells are separated and flow into different outlets under the action of the AC-DIEP fo...

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Abstract

The invention provides a blood cell microfluidic separation device and method combining magnetophoresis and dielectrophoresis. The device comprises a microchannel, two liquid inlets, four liquid outlets, a permanent magnet, a ferromagnet and two pairs of microelectrodes. The device is sequentially provided with a magnetophoresis separation area and a dielectrophoresis separation area according to the flowing direction of a sample, the magnetophoresis separation area generates a magnetic field through a permanent magnet, and separation of a red blood cell and blood platelet mixed sample and a circulating tumor cell and white blood cell mixed sample is achieved. The dielectrophoresis separation area utilizes an asymmetric hole structure to generate a high-gradient non-uniform electric field so that separation of red blood cells and blood platelets and separation of circulating tumor cells and white blood cells are realized respectively. Magnetic electrophoresis is used as a pre-enrichment stage of dielectrophoresis separation to realize primary separation, and dielectrophoresis is used for realizing secondary separation so that continuous separation of circulating tumor cells, red blood cells, blood platelets and white blood cells in a blood sample is realized, the cell sample does not need to be marked in advance, and the physiological activity of cells is not influenced.

Description

technical field [0001] The invention relates to the technical field of cell separation, in particular to a blood cell microfluidic separation device and method combining magnetophoresis and dielectrophoresis. Background technique [0002] Isolation of cells is an essential step in cell biology research and many diagnostic and therapeutic methods. Because blood is an extremely informative, readily accessible tissue, many current diagnostic tests rely on isolated blood components, such as white blood cells, which are required for several hematology tests and for DNA sequencing. Blood components are also used in therapeutics, and transfusions of purified platelets are often required during surgery. Some special cells in the blood often indicate a certain disease. For example, mononuclear red blood cells are a marker of diseases related to abnormal conversion of red blood cells. Another example is that circulating tumor cells, a rare cell, can be found in patients before the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
CPCB01L3/502753B01L3/50273B01L2400/0424B01L2400/043
Inventor 王俊生赵鹏璐陈斌赵迅赵凯
Owner DALIAN MARITIME UNIVERSITY
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