Method and system for multi-layer autofocus
An automatic focus and focal plane technology, applied in the field of image processing, can solve the problems of system judgment error, strong focus position, unable to focus on the target object, etc., to achieve the effect of accurate focus
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[0049] see figure 1 , figure 1 A multi-layer autofocus system is shown, the system includes: a light source 1, a collimating lens 2, a semicircular diaphragm 3, a half mirror 4, a microscope objective 5, an object stage 6, a focusing Lens 7 , area array detector 8 and data processing unit 9 .
[0050] The light source 1 adopts a laser light source, which is arranged above the collimating lens and is used for emitting a laser beam to the collimating lens 2 .
[0051] In one possible design, a laser light source with a wavelength of 850 nm is used, which belongs to the biological and eye-safe band.
[0052] The collimating lens 2 is used to expand the laser beam, so that the diameter of the laser beam is larger than the diameter of the microscope objective lens 5; the collimating lens 2 collimates the laser beam.
[0053] In a possible design, the collimating lens 2 expands the laser beam to a diameter of 16 mm, so that the diameter of the laser beam is larger than the diamet...
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