Method for rapidly and efficiently obtaining callus of Scadoxusmultiflorus subsp. multiflorus
A technology of callus and fireball, which is applied in the field of plant tissue culture, can solve the problems of limited reproduction number, danger of wild population entry, and inability to meet social production, and achieve the effect of strong tissue meristem ability and great dedifferentiation potential
Active Publication Date: 2021-08-20
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
View PDF1 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0005] There is a small amount of introduced and cultivated species in our country, but it is far from meeting the actual needs of social production, and a large number of multiplication is particularly important.
At present, the propagation methods commonly used in the world for this species are seed propagation and division (ball) propagation, but the number of reproduction of these two propagation methods is extremely limited, and it is inevitable to dig a large number of wild plants, and the wild population may enter danger. status
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0021] Grab the underground part of an adult plant of Scadoxus multiflorus subsp. multiflorus in 3-4 years, and select the branches (lateral rhizomes) that grow from the mother plant but do not emerge from the ground, such as figure 1 shown. Select the stem segment with a length of 3-8cm at the top of the branch, such as figure 1 Wash as indicated by the yellow arrow. Then remove the about 1cm long stem segment apex of the top, and the remaining part is cut into 0.5-0.8cm long segments to obtain explants, which are the lateral rhizomes described in Table 1. At the same time, get the leaf tissue (including leaf tip, leaf margin and leaf vein), cut into 0.5-0.8cm long small cubes, as the explant of leaf; get the middle part of the pseudostem, cut into 0.5-0.8cm long small Cube, as explant of pseudostem; take 1-3cm of root tip, cut into 0.5-0.8cm long section, as explant of root; strip off the outer layer of corm that has been sterilized, leaving only the "core" Part, cut this...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a method for rapidly and efficiently obtaining the callus of Scadoxusmultiflorus subsp.multiflorus. The method specifically comprises the following steps: grabbing the corm of the 3-4-year-old Scadoxusmultiflorus subsp. multiflorus, selecting a 3-8 cm stem section at the top end of a lateral subterraneous stem, performing cleaning, removing a 0.5-1 cm long tip end of the stem section at the top end, cutting the remaining part into small sections with the length of 0.5-0.8 cm as explants, carrying out culturing in a callus induction culture medium containing MS, 2.4-D with the concentration of 0.45-0.48 mg / L and NAA with the concentration of 0.05-0.08 mg / L, and obtaining the callus. Compared with division propagation and sowing propagation, the method has the advantages that stock plants are not damaged, many seedlings are produced at a time, large-scale production is facilitated, and the method is suitable for large-scale propagation.
Description
technical field [0001] The invention belongs to the field of plant tissue culture, in particular to a method for rapidly and efficiently obtaining callus of Scadoxus multiflorus subsp. multiflorus. Background technique [0002] Scadoxus multiflorus subsp. multiflorus is native to a wide area of sub-Saharan Africa, from Senegal to Somalia, and from Ethiopia to South Africa. [0003] It is a perennial herb, 15-120cm tall, bulbous, with petioles forming pseudostems 5-60cm high. The leaves are lanceolate to obovate, acute to acuminate or obtuse, the base of the leaf gradually narrows to nearly heart-shaped, and the size of the leaves varies greatly; flowers first and then leaves. Scapes 12-75cm tall, pseudobulbs and scapes usually have maroon to dark purple spots. Involucral bracts droop or wither early, lanceolate to linear, up to 6cm long, less than 1.5cm wide, colorless or slightly reddish. Inflorescence hemispherical to spherical, 10-200 flowers. The peduncle is 1-6.5c...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 钟志祥王青锋严雪胡光万刘艳玲李洪林
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI