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Antigen peptide (neoantigen) related to liver cancer driver gene mutation and application thereof

A technology of driving genes and antigenic peptides, applied in the field of biomedicine, can solve the problems of failure to form effects, superior therapeutic agents, etc., and achieve good clinical transformation and disease prevention prospects

Active Publication Date: 2021-08-31
上海保亿仁生物医药有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, due to the need for multidisciplinary, high-tech, and multi-platform collaborative research, the antigenic peptides related to liver cancer driver gene mutations have not been systematically discovered and a relatively complete peptide library has not been established, so it has not yet formed a superior effect. therapeutic agents, etc.

Method used

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  • Antigen peptide (neoantigen) related to liver cancer driver gene mutation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] Below, in conjunction with specific embodiment, the present invention is described further:

[0020] Antigen peptides related to liver cancer driver gene mutations used in the examples are shown in Table 1:

[0021] Table 1 Antigen peptide sequences related to liver cancer driver gene mutations

[0022]

[0023] The operation for the establishment of antigenic peptide-specific CTL clones related to liver cancer driver gene mutations is as follows:

[0024] 10 from the same healthy donor 5 CD8 + T cells by loading 10 antigenic peptides associated with liver cancer driver gene mutations 4 Mo-DCs were stimulated twice at intervals of 1 week, and then passed autologous 10 5 Mitomycin C-treated PBMCs loaded with antigenic peptides associated with liver cancer driver gene mutations were stimulated once and screened by standard cytotoxicity assays.

[0025] T2 cells were loaded with 5uM antigen peptides related to liver cancer driver gene mutations as target cells, and...

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Abstract

The invention discloses an antigen peptide (neoantigen) related to liver cancer driver gene mutation. A liver cancer driver gene is at least two of CDKN2A, ERBB4, HNF1A, NFE2L2, NTRK3, LRP1B, PREX2, ZFHX3 and ZNF521; the sequences of the antigen peptide are at least two of SEQ No.1-17; according to the application of the antigen peptide, the antigen peptide is used for inducing generation of specific cytotoxic T cell clone; and the antigen peptide has high affinity with MHC I molecules on DC cells, can effectively stimulate and induce generation of specific cytotoxic T lymphocytes, can be used for immune clearance of tumor cells with driver gene mutation related to liver cancer, and has good treatment potential.

Description

technical field [0001] The invention relates to an antigenic peptide related to liver cancer driver gene mutation and application thereof, belonging to the technical field of biomedicine. Background technique [0002] Liver cancer is a malignant tumor of the liver, which can be divided into two categories: primary and secondary. Primary hepatic malignant tumors originate from the epithelial or mesenchymal tissues of the liver. The former is called primary liver cancer. [0003] Treatment methods include surgery, hepatic artery ligation, hepatic arterial chemoembolization, radiofrequency, cryotherapy, laser, microwave, as well as chemotherapy and radiation therapy. Biological therapy and traditional Chinese medicine are also widely used in the treatment of liver cancer. [0004] With people's understanding of the nature of the immune response, the concept of cellular immunity was proposed, which uses the body's natural defense system to selectively destroy tumor cells throu...

Claims

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Application Information

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IPC IPC(8): C07K14/47C12N5/0783A61K39/00A61P35/00
CPCA61K39/0011A61P35/00A61K2039/5154C07K14/47C12N5/0636
Inventor 郝冰娜李许峰蔡睿赵环罗夫辛克纳吉张积仁
Owner 上海保亿仁生物医药有限公司
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