Method for analyzing relation between mountain soil fungal species and carbon metabolism by combining high-throughput sequencing technology and Biolog method

A technical analysis and high-throughput technology, applied in the field of analyzing the relationship between mountain soil fungal species and carbon metabolism, can solve the problem of inability to accurately detect the correlation between component species and transgenic crops

Pending Publication Date: 2021-09-03
QUANZHOU NORMAL UNIV
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Problems solved by technology

The disadvantage of this method is that it can only analyze the effect of transgenic crops on the function of the overall microbial community in the rhizosphere soil, but cannot accurately detect the correlation between the main constituent species of soil microorganisms and transgenic crops

Method used

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  • Method for analyzing relation between mountain soil fungal species and carbon metabolism by combining high-throughput sequencing technology and Biolog method
  • Method for analyzing relation between mountain soil fungal species and carbon metabolism by combining high-throughput sequencing technology and Biolog method
  • Method for analyzing relation between mountain soil fungal species and carbon metabolism by combining high-throughput sequencing technology and Biolog method

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Experimental program
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Embodiment 1

[0028] Example 1

[0029] (1) Sampling

[0030] Taking the soil of Wuyishan National Park in Fujian Province as the research object, high-throughput sequencing and Biolog methods were used to compare the correlation between the composition of dominant species and the metabolism of six types of carbon sources at the fungal genus level, and to study the relationship between fungal species and carbon source metabolism in mountain soils .

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Abstract

The invention provides a method for analyzing a relation between mountain soil fungal species and carbon metabolism by combining a high-throughput sequencing technology and a Biolog method. According to the method, a high-resolution fungal species genus level composition is clarified by using the high-throughput sequencing technology, the utilization rate of microbial communities on six types of carbon sources is analyzed by using the Biolog method, and then the correlation between the species composition under the fungal genus level and the six types of carbon sources is clarified by using a rank analysis method. Through the analysis, the difference of the dominant species composition on the metabolic capability of various carbon sources under the fungal genus level is intuitively reflected, and the mutual relation between the dominant species composition and the carbon source metabolism under the fungal genus level in a specific region is defined. A basis is provided for virtuous circle and reasonable use of a mountain soil ecosystem.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for analyzing the relationship between fungal species and carbon metabolism in mountain soil by combining high-throughput sequencing and Biolog method. Background technique [0002] Microorganisms are an important part of mountain soil ecosystems. They dominate the nutrient cycle and energy flow of mountain soil ecosystems, and affect soil activity and soil fertility by participating in soil formation and development, material transformation, and energy transfer. However, fungi in mountain soils can respond to organic matter formed by soil litter more quickly than bacteria, and preferentially decompose macromolecular compounds, which is extremely important for carbon source metabolism and organic matter cycle in mountain soil ecosystems. Only by finding species closely related to carbon metabolism in the huge mountain soil fungal system can we better u...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/06C12Q1/04
CPCC12Q1/6895C12Q1/06C12Q1/04C12Q2600/158C12Q2600/178
Inventor 张秋芳黄卫红王俊奇袁建军黄兆斌
Owner QUANZHOU NORMAL UNIV
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