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An antibacterial metal nanoenzyme oa-mno 2 The preparation method and application of

A technology of antibacterial metals and nanozymes, which is applied in antibacterial drugs, nanotechnology, nanotechnology, etc., can solve the problems of limiting nanomaterials to artificially simulate enzymes, uneven distribution of nanoparticles, and complex steps of nanomaterials, so as to promote skin wound healing. Healing, wide range of raw material sources, high catalytic efficiency

Active Publication Date: 2022-03-25
CHANGZHOU UNIV
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  • Abstract
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Problems solved by technology

[0004] Although nanozymes have made great progress, the low catalytic activity and poor stability limit the development of artificial enzymes of nanomaterials. The poor stability is mainly due to the uneven distribution of nanoparticles and easy aggregation. The low catalytic activity is mainly due to the impact on the catalytic environment. There are certain requirements, for example: gold nanoparticles exhibit inherent catalase-like catalytic activity only under alkaline conditions, while the microenvironment of the bacterial infection site is weakly acidic
In addition, the preparation of stable nanomaterials often requires relatively complex steps, which limit the further application of nanozymes in medical science.

Method used

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  • An antibacterial metal nanoenzyme oa-mno <sub>2</sub> The preparation method and application of
  • An antibacterial metal nanoenzyme oa-mno <sub>2</sub> The preparation method and application of
  • An antibacterial metal nanoenzyme oa-mno <sub>2</sub> The preparation method and application of

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Embodiment 1

[0053] 1. OA-MnO 2 Synthesis and purification of nanozymes

[0054] Dissolve 200 μL of oleic acid in 7 mL of absolute ethanol, add it into 7 mL of water in a water bath at 60 ° C, and stir vigorously for 5 min. At this time, a milky white homogeneous oleic acid nanoemulsion is formed, and immediately cooled in an ice bath.

[0055] In order to find out the preparation of OA-MnO 2 Weigh 10, 20, 30, 40, 50, 60, and 70 mg of potassium permanganate solids, dissolve them in 5 mL of water, and quickly inject them into the vigorously stirred oleic acid emulsion. The white emulsion changes from violet to wine red, continue to stir until the solution turns brown, at which point a stable OA-MnO 2 Nanoparticles, when reduced 70mg KMnO 4 At this time, the phenomenon of nanoparticle aggregation occurs, continue to stir for 1 hour and dialyze (MV=14000), and change the water every 6 hours until the volume of the solution does not change any more.

[0056] 2. OA-MnO 2 Characterization o...

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Abstract

The invention belongs to the technical field of metal nanomaterials, and specifically discloses an antibacterial metal nanoenzyme OA‑MnO 2 preparation methods and applications. One-pot synthesis of manganese dioxide (OA‑MnO) via oleic acid (OA) nanoemulsion template method 2 ) nanozyme. It has good oxidase activity, can generate reactive oxygen species (ROS), and cause microbial cell damage, thereby effectively killing Gram-positive (Staphylococcus aureus) and negative (Escherichia coli) strains, and is effective against Staphylococcus aureus Has good biofilm prevention and destruction capabilities. Antibacterial metallo-nanozyme OA-MnO prepared by the present invention 2 It exhibits high stability and biocompatibility under physiological conditions, and is superior to vancomycin in antibacterial properties in vivo. It is a safe and effective tool for fighting bacterial infections and promoting wound healing.

Description

technical field [0001] The invention belongs to the technical field of metal nanomaterials, in particular to an antibacterial metal nanozyme OA-MnO 2 preparation methods and applications. Background technique [0002] Natural enzymes have strong catalytic activity under mild conditions, and exist in various processes of organism life activities, such as: tricarboxylic acid cycle, glycolysis, synthesis and metabolism of lipids, proteins and amino acids, etc., most of the natural enzymes The main component of the enzyme is protein, which has strict requirements on the reaction environment. The protein is easily denatured in the environment of overacidity, overalkaline, overcooling and overheating, and the catalytic activity of the enzyme is also lost. As a result, artificial mimetic enzymes have been produced, and metal nanozymes are one of them. [0003] Nanozymes are nanomaterials capable of catalyzing biological substrates with reaction kinetics similar to natural enzymes...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K33/32A61K47/54A61K47/69A61P31/04A61P17/00A61P17/02C01G45/02B82Y40/00B82Y5/00
CPCA61K33/32A61K47/542A61K47/6929A61P31/04A61P17/00A61P17/02C01G45/02B82Y40/00B82Y5/00C01P2004/64C01P2004/62C01P2006/22Y02A50/30
Inventor 王程刘丽李颖惠泽轩高子翰王建浩崔朋飞周舒文邱琳
Owner CHANGZHOU UNIV
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