Cobaltosic oxide nano flower ball, preparation method and various mimic enzyme activity applications

A nano-technology of cobalt tetroxide, which is applied in the field of cobalt tetroxide nano flower bulbs and preparation, can solve the problem of single imitation enzyme activity, etc., and achieve the effect of simple and effective preparation method

Inactive Publication Date: 2020-06-26
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the previous study of the inventors of the present invention, at present, most of the reported nanomaterials only have a single imitation enzyme activity, such as imitation oxidase activity, imitation peroxidase activity, imitation catalase activity or imitation superoxidase activity. dismutase activity, etc.
On the other hand, only a few nanozymes have a variety of imitation enzyme activities, but most of them are composite materials containing multiple metal elements or require high temperature treatment during the preparation process, so how to synthesize a variety of imitation enzymes simply and effectively Enzyme-active nanomaterials have become a major challenge

Method used

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  • Cobaltosic oxide nano flower ball, preparation method and various mimic enzyme activity applications
  • Cobaltosic oxide nano flower ball, preparation method and various mimic enzyme activity applications
  • Cobaltosic oxide nano flower ball, preparation method and various mimic enzyme activity applications

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Embodiment approach

[0042]The third embodiment of the present invention provides an application of the above-mentioned cobalt trioxide nanocurds in biosensing, tumor drug, biocatalyst and / or environmental monitoring.

[0043] The fourth embodiment of the present invention provides a method for catalyzing the oxidation and color development of 3,3',5,5'-tetramethylbenzidine (TMB). ',5,5'-Tetramethylbenzidine in acetate buffer, and then reacted.

[0044] In one or more examples of this embodiment, the pH of the acetate buffer is 4.3-4.7.

[0045] In one or more examples of this embodiment, the addition ratio of cobalt trioxide nanocurds to 3,3',5,5'-tetramethylbenzidine is 15-25:1, μg:mol.

[0046] In one or more examples of this embodiment, the reaction temperature is 23-27°C.

[0047] The fifth embodiment of the present invention provides a method for catalyzing the color development of 3,3',5,5'-tetramethylbenzidine catalyzed by hydrogen peroxide. and 3,3',5,5'-tetramethylbenzidine in acetate...

Embodiment 1

[0061] Co with multi-enzyme activity 3 o 4 Preparation of nanozymes:

[0062] Freshly prepared ice-cold 10 mM (final concentration, the same below) sodium borohydride aqueous solution was added dropwise to 2 mM cobalt chloride aqueous solution. After overnight stirring at room temperature, the pellet was centrifuged and washed 3 times. Co obtained after freeze-drying 3 o 4 Nano flower balls. Will Co 3 o 4 Prepared into a 1mg / mL solution for use. figure 1 Co observed by transmission electron microscopy 3 o 4 TEM imaging. figure 2 for Co 3 o 4 X-ray photoelectron spectroscopy analysis diagram.

Embodiment 2

[0064] co 3 o 4 Oxygenase Activity Verification of Nanozymes:

[0065] Experimental system a: The catalytic reaction system is composed of acetate buffer (pH 4.5, 100mM), Co 3 o 4 (10μg / mL) and TMB (0.5mM) were reacted at 25°C for 10 minutes, and the visible absorption spectrum in the range of 400–800nm ​​was recorded by a microplate reader.

[0066] Another control experiment b: the catalytic system contains acetate buffer (pH 4.5, 100mM) and the Co obtained in the above-mentioned Example 1 3 o 4 (10 μg / mL), the remaining reaction conditions are the same as the above-mentioned experimental system, and the absorbance value is measured after 10 minutes.

[0067] Another control experiment c: the Co obtained in the above-mentioned Example 1 is not added to the catalytic reaction system 3 o 4 , after reacting for 10 minutes under the same conditions as the above-mentioned experimental system, the absorbance value was detected.

[0068] Such as image 3 As shown, the expe...

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Abstract

The invention discloses a cobaltosic oxide nano flower ball, a preparation method and various mimic enzyme activity applications. The preparation method comprises the following steps: dropwise addinga sodium borohydride solution into a bivalent cobalt salt solution to carry out an overnight reaction, and then drying. The cobaltosic oxide nano flower ball prepared by the method has four kinds of biomimetic activity at the same time, including oxidase activity, peroxidase activity, catalase activity and superoxide dismutase activity.

Description

technical field [0001] The invention belongs to the field of nanomaterial preparation and enzymology, and relates to a cobalt trioxide nano-curd, a preparation method and various imitative enzyme activity applications. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Compared with natural biological enzymes, nano-mimetic enzymes have attracted more and more attention in recent years because of their advantages of various enzyme-mimicking activities, low cost, high catalytic activity, easy large-scale preparation and good stability. . Due to the excellent characteristics of nanozymes, they have potential applications in biochemical analysis, biosensors, environmental monit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01G51/04B82Y40/00B01J35/08B01J23/75
CPCB01J23/75B01J35/08B82Y40/00C01G51/04C01P2002/85C01P2004/04C01P2004/32
Inventor 刘爱骅刘璇颜露
Owner QINGDAO UNIV
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