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Method for inducing peronophythora litchii sporangia to release zoospores

A technology for Phytophthora lychee and zoospores is applied in the field of microbial culture, which can solve the problems of long sporulation time, low sporulation yield, and inability to obtain zoospores in large quantities, and achieves a short induction period and a simple method. Effect

Active Publication Date: 2021-09-10
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the present invention proposes a method for inducing the sporangia of Peronospora litchie to release zoospores to solve the above-mentioned low sporulation yield, long sporulation time, sporangia are difficult to concentrate and release zoospores, which cannot meet the requirements of large quantities. The problem with getting zoospores

Method used

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  • Method for inducing peronophythora litchii sporangia to release zoospores
  • Method for inducing peronophythora litchii sporangia to release zoospores
  • Method for inducing peronophythora litchii sporangia to release zoospores

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Embodiment 1

[0026] Method for inducing spores of spore sac in lychee cream hyphae release swim: including the following steps:

[0027] (1) The production of spore bladder of the lychee cream is: 0.3 × 0.3 cm lychee cream pyrophylosis in the sterile workbench is inoculated into a culture dish equipped with a culture medium and having a diameter of 11 cm, cultured under dark conditions. 5D. Placed in the light 700LUX, the temperature is 23 ° C for continuous culture 1D, producing a phosphocystosporia phenol; the preparation of the culture fluid is 8 parts of V8 vegetable juice, 1 windproof grass juice, 2 icon fruit peel polyphenols, two The yeast fermented product was 0.3 parts of the porous matter, 3 parts of agar powder, and mixed with 80 parts of deionized water for 5 min, and then 20 min at 100 ° C;

[0028] (2) Inducing generation of swantoenes, the culture dish in step (1) is filled with mycelium, and the sterile L-type glass stick is applied to the plate, and then placed in 20 ° C. 20h,...

Embodiment 2

[0032] Method for inducing spores of spore sac in lychee cream hyphae release swim: including the following steps:

[0033] (1) The production of spore bladder of the lychee cream is: 0.3 × 0.3 cm lychee cream pyrophylosis in a sterile workbench is seeded in a culture dish equipped with a culture medium and having a diameter of 11 cm, and cultured under dark conditions 7d under dark conditions. , Placed in the light 900LUX, the temperature is 30 ° C for 3D, resulting in a phosphocystosis of the lychee cream hyphae; the preparation of the culture fluid is 15 parts of V8 vegetable juice, 6 windswear juice, 10 icon fruit peel polyphenols, two molt The fermentation product was less than 1.2 parts, 8 parts of agar powder, 100 parts of deionized water for 30 min, and then sterilized at 130 ° C high pressure steam sterilization;

[0034] (2) Inducing generation of swantoenes: The petri dish in step (1) is filled with mycelium, and the sterile L-type glass stick is applied to the plate, a...

Embodiment 3

[0038] Method for inducing spores of spore sac in lychee cream hyphae release swim: including the following steps:

[0039](1) generating lychee Peronophythora sporangia: sterile workbench 0.5 × 0.5cm cut litchi block Peronophythora broth and inoculated into 11cm diameter petri dishes, cultured under dark conditions 5d and placed in a light 800lux, continuous culture at temperature 25 ℃ 2d, litchi produced sporangia Peronophythora; liquid culture is prepared to 8 parts by V8 vegetable juice, juice 1 part by wind roots, 2 parts litchi pericarp polyphenols, bifid The product was dissolved yeast fermentation spore 0.3 parts agar powder 3 parts, 80 parts of deionized water were mixed and stirred 18min, and then to steam sterilization at 121 ℃ 20min;

[0040] (2) induction of sporangia produced: After step dish full of hyphae production (1), sterile L-plate covered with a glass rod applicator mycelium, and then placed in a 25 deg.] C standing light 800lux culture 24h, can induce sporan...

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Abstract

The invention provides a method for inducing peronophythora litchii sporangia to release zoospores. The method comprises the following steps of generation of the peronophythora litchii sporangia, induced generation of zoosporangia, centralized release of zoospores, collection of the zoospores, preparation of a nutrient solution and setting of a cultivation environment. According to the method, the optimum conditions for inducing peronophythora litchii to generate the sporangia and intensively releasing the zoospores can be achieved, the induction of a large number of zoosporangia and the centralized release of the zoospores can be achieved in a short time, the obtained sporangia are large in forming amount, the zoospores are high in release rate and good in germination rate, the problem that the zoospores are difficult to intensively release in a common method is solved, and the problem that zoosporangia generated by induction of a water culture method are easy to pollute is also avoided.

Description

Technical field [0001] The present invention relates to the field of microbial culture techniques, and more particularly to a method of inducing a spore sac in fluctuating spore sac in lychee cream. Background technique [0002] Percheythora, Litchi, also known as lychee cream mildew, lychee disease, its pathogen is a pebling granter, hyperi, is one of the main diseases of lychee. The disease was first found in Taiwan in China in 1934, later discovered that this disease was discovered in the mainland of China's Litchi, Australia, Thailand, Vietnam, Babu New Guinea. The disease is not only harmful to the crowd fruit, but also harms the henaband, the nuts, the results, and the fruits caused a large number of falling fruits. At the beginning of the disease, the victims were brown, and the fruit was dark, the fruit was dark brown; if the disease is in the middle of the rain, the flowers, the fruit, etc., the surface of the disease is full of white frost. Litchi cream disease often ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N3/00C12R1/645
CPCC12N1/14C12N3/00
Inventor 陈庆河杨成东吕林贾斯蒂
Owner HAINAN UNIVERSITY
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