Extraction method of China-hemp seed storage protein
A technology for protein storage and extraction method, which is applied to the protein composition of vegetable seeds, protein food ingredients, food science, etc., can solve problems such as poor effect, achieve obvious banding, reduce labor costs, and save fine separation and purification. effect of time
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[0013] Embodiment 1: The extraction method of hemp seed storage protein of the present embodiment is carried out according to the following steps:
[0014] 1. Extraction: Grind hemp seeds into powder, add n-hexane to the powder in a ratio of 1:4 by weight of hemp seeds, extract at room temperature for 3 hours to obtain an extract, and then according to the ratio of hemp seeds by weight In a ratio of 1:6, n-hexane was added to the extract, and after standing overnight, the n-hexane was dried to obtain defatted hemp powder;
[0015] 2. Add deionized water to the defatted hemp powder in a ratio of 1:10 in parts by weight, shake and extract at 37°C for 1 hour, then place at room temperature, and then centrifuge at 8000g for 30 minutes to obtain the above The supernatant liquid A and the precipitate A were added into the precipitate A with deionized water in a ratio of 1:5 by weight. Liquid B and precipitation B, supernatant liquid A and supernatant liquid B are combined to obtain...
Example Embodiment
[0017] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the hemp seeds in step 1 are ground into powder by using liquid nitrogen. Other steps and parameters are the same as in the first embodiment.
[0018] In this embodiment, fresh hemp seeds are ground into as fine powder as possible with liquid nitrogen.
Example Embodiment
[0019] Embodiment 1 utilizes the method of the present invention to extract the storage protein of different components in the hemp seeds of Heilongjiang Province, and concrete steps are as follows:
[0020] 1. Extraction: Grind hemp seeds into powder, add n-hexane to the powder in a ratio of 1:4 by weight of hemp seeds, extract at room temperature for 3 hours to obtain an extract, and then according to the ratio of hemp seeds by weight In a ratio of 1:6, n-hexane was added to the extract, and after standing overnight, the n-hexane was dried to obtain defatted hemp powder;
[0021] 2. Add deionized water to the defatted hemp powder (hemp seeds are ground into powder with liquid nitrogen) in a ratio of 1:10 in parts by weight, shake and extract at 37°C for 1 hour, and then leave at room temperature. Centrifuge at 8000g for 30 minutes to obtain supernatant A and precipitate A, add deionized water to precipitate A according to the weight ratio of 1:5, shake and leaching at 37°C f...
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