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Method for extracting astaxanthin from phaffia rhodozyma

A technology of Phaffia yeast and astaxanthin, applied in the direction of organic chemistry, can solve the problems of low extraction rate of astaxanthin and large loss of astaxanthin, and achieve the effect of improving extraction rate and reducing loss

Active Publication Date: 2021-09-24
浙江皇冠科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods all have the defects of low astaxanthin extraction rate and large astaxanthin loss in varying degrees.

Method used

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  • Method for extracting astaxanthin from phaffia rhodozyma
  • Method for extracting astaxanthin from phaffia rhodozyma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Accurately weigh 1g of Phaffia yeast with an electronic balance, add 15ml of dimethyl sulfoxide, and soak at 32°C for 15 minutes; centrifuge to obtain soaked Phaffia yeast; add 5ml of 75% ethanol to the soaked Phaffia yeast , sonicate for 45s, control the ultrasonic amplitude to 60 μm, and centrifuge to obtain Phaffia cell fragments; add 40ml of acetone to the Phaffia cell fragments, extract at 60°C for 15min, and obtain an astaxanthin extract. The calculated astaxanthin yield was 195.3 μg / g.

Embodiment 2

[0047] Accurately weigh 1g of Phaffia yeast with an electronic balance, add 12ml of dimethyl sulfoxide, and soak at 35°C for 10 minutes; centrifuge to obtain soaked Phaffia yeast; add 4ml of 75% ethanol to the soaked Phaffia yeast , sonicate for 35s, control the ultrasonic amplitude to 48μm, and centrifuge to obtain Phaffia cell fragments; add 45ml of acetone to the Phaffia cell fragments, and extract at 50°C for 12min to obtain astaxanthin extract. The calculated astaxanthin yield was 180.7μg / g.

Embodiment 3

[0049] Accurately weigh 1g of Phaffia yeast with an electronic balance, add 18ml of dimethyl sulfoxide, and soak at 30°C for 12 minutes; centrifuge to obtain soaked Phaffia yeast; add 4.5ml of 75% Phaffia yeast to the soaked Phaffia yeast Ethanol, ultrasonic treatment for 30s, control the ultrasonic amplitude to 60 μm, and centrifuge to obtain Phaffia cell fragments; add 50ml of acetone to the Phaffia cell fragments, and extract at 52°C for 10min to obtain astaxanthin extract. The calculated astaxanthin yield was 182.6 μg / g.

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Abstract

The invention relates to the technical field of astaxanthin extraction methods, and provides a method for extracting astaxanthin from phaffia rhodozyma. The method comprises the steps of (1) carrying out soaking pretreatment on phaffia rhodozyma by using dimethyl sulfoxide, and then separating the phaffia rhodozyma; (2) adding ethanol into the separated phaffia rhodozyma, and carrying out wall breaking treatment by using ultrasonic waves; (3) carrying out solid-liquid separation to obtain phaffia rhodozyma cell debris; and (4) adding acetone into the phaffia rhodozyma cell debris for extraction to obtain an astaxanthin extract. The invention provides a method for extracting astaxanthin from phaffia rhodozyma, which adopts a two-step wall breaking method, namely, a dimethyl sulfoxide wall breaking method and an ultrasonic wall breaking method, to treat the phaffia rhodozyma in sequence, so that the extraction rate of the astaxanthin is increased, the loss of the astaxanthin is reduced, and the yield of the astaxanthin can reach 180.7-197.6 mu g / g.

Description

technical field [0001] The invention relates to the technical field of astaxanthin extraction methods, in particular to a method for extracting astaxanthin from Phaffia yeast. Background technique [0002] Among the dozen or so carotenoids produced by Phaffia yeast, astaxanthin is the main component. In vivo, astaxanthin has various biological activities such as strong anti-oxidation, anti-tumor, and anti-photosensitivity, so it plays an important role in food, cosmetics, pharmaceuticals, and aquaculture industries. However, astaxanthin is the intracellular pigment of Phaffia yeast, and the wall-breaking effect and extraction method directly affect the yield and quality of the pigment. Therefore, it is of great significance for the industrial production and application of astaxanthin to find an effective method of breaking and extracting Phaffia yeast. At present, the commonly used yeast wall breaking methods include autolysis method, acid method, alkali method, dimethyl s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24
CPCC07C403/24C07C2601/16
Inventor 胡向东梁新乐
Owner 浙江皇冠科技有限公司