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Marker combination and application thereof in mitosis recombination hotspot detection

A technology of mitosis and tag combination, applied in the field of synthetic biology, can solve the problems of insufficient research on yeast recombination hotspots and cold spots, screening of restricted recombination strains, etc.

Active Publication Date: 2021-10-22
TIANJIN UNIV
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Problems solved by technology

[0006] Although a lot of research has been carried out on the genomic changes of yeast mitotic recombination, due to the limitation of the construction of mitotic library and the screening of recombinant strains, the research on the hot and cold spots of yeast recombination is not enough.
However, a suitable PCRTag watermark label has not been found for the study of mitotic recombination hotspots

Method used

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  • Marker combination and application thereof in mitosis recombination hotspot detection
  • Marker combination and application thereof in mitosis recombination hotspot detection
  • Marker combination and application thereof in mitosis recombination hotspot detection

Examples

Experimental program
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Effect test

Embodiment

[0047] 1. Construction of rearrangement strain library

[0048] 1.1 Strains

[0049] ① The diploid Saccharomyces cerevisiae strain yLHM120 was used as the starting strain, which contained a wild-type V chromosome (wtV) and a synthetic V chromosome (synV), and was from Tianjin University.

[0050] ②Triploid Saccharomyces cerevisiae strain yXZX1892 was used as the starting strain, which contained one wild-type V chromosome (wtV) and two synthetic V chromosomes (synV), and was from Tianjin University.

[0051] ③Tetraploid Saccharomyces cerevisiae strain yLHM086 was used as the starting strain, which contained two wild-type V chromosomes (wtV) and two synthetic V chromosomes (synV), and was from Tianjin University.

[0052] 1.2 Induced rearrangement:

[0053] A single colony carrying pCRE4 was picked from the SC-His plate, inoculated into 3 mL of SC-His medium, and cultured with shaking at 30°C for 24 hours.

[0054] Centrifuge at 5000 rpm for 2 min at room temperature, remove ...

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Abstract

The invention relates to the technical field of synthetic biology, in particular to a marker combination and application thereof in mitosis recombination hotspot detection. The PCRTag tag combination provided by the invention can be used for rapidly detecting eukaryotic genome mitosis recombination hotspots. By utilizing the tag combination, the mitosis recombination hot spot of the homologous chromosome can be quickly identified through the specific watermark tag, and a convenient method is provided for researching the eukaryotic mitosis recombination event.

Description

technical field [0001] The invention relates to the technical field of synthetic biology, in particular to a marker combination and its application in the detection of mitotic recombination hotspots. Background technique [0002] Homologous recombination between DNA molecules refers to the biological process in which DNA sequences undergo double-strand breaks, recombination repair, and chromosome separation processes to achieve recombination between sequences and form new DNA molecules. Homologous recombination can not only repair the double-strand break (DSB) of DNA and maintain genome stability, but also realize the cross-exchange of genetic information, generate genetic diversity of daughter cells, and accelerate the process of biological evolution. [0003] Homologous recombination includes two ways of meiotic recombination and mitotic recombination. In the natural state, the genetic material in cells is more inclined to undergo meiotic recombination. The incidence of mi...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12R1/865
CPCC12Q1/6895C12Q2600/156
Inventor 元英进谢泽雄付娟谢心妍
Owner TIANJIN UNIV
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