Marker combination and application thereof in mitosis recombination hotspot detection

A technology of mitosis and tag combination, applied in the field of synthetic biology, can solve the problems of insufficient research on yeast recombination hotspots and cold spots, screening of restricted recombination strains, etc.
CN113528699AActive Publication Date: 2021-10-22TIANJIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2021-10-22

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Abstract

The invention relates to the technical field of synthetic biology, in particular to a marker combination and application thereof in mitosis recombination hotspot detection. The PCRTag tag combination provided by the invention can be used for rapidly detecting eukaryotic genome mitosis recombination hotspots. By utilizing the tag combination, the mitosis recombination hot spot of the homologous chromosome can be quickly identified through the specific watermark tag, and a convenient method is provided for researching the eukaryotic mitosis recombination event.
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Description

technical field

[0001] The invention relates to the technical field of synthetic biology, in particular to a marker combination and its application in the detection of mitotic recombination hotspots. Background technique

[0002] Homologous recombination between DNA molecules refers to the biological process in which DNA sequences undergo double-strand breaks, recombination repair, and chromosome separation processes to achieve recombination between sequences and form new DNA molecules. Homologous recombination can not only repair the double-strand break (DSB) of DNA and maintain genome stability, but also realize the cross-exchange of genetic information, generate genetic diversity of daughter cells, and accelerate the process of biological evolution.

[0003] Homologous recombination includes two ways of meiotic recombination and mitotic recombination. In the natural state, the genetic material in cells is more inclined to undergo meiotic recombination. The incidence of mi...

Claims

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