Application of gold nanoclusters and medicine for relieving male reproductive toxicity of copper ions
A gold nanocluster, male reproduction technology, applied in the field of biomedicine, can solve problems such as increasing excretion, and achieve the effects of improving absorption rate, reducing death, and enhancing targeting.
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Embodiment 1
[0024] Example 1: Gold nanoclusters specifically absorb copper ions
[0025] Specific steps:
[0026] AuNCs were first synthesized: in one embodiment, chloroauric acid (5 mL, 10 mmol / L) and BSA (5 mL, 50 mg / mL) were stirred at 37°C for 2 minutes. Then add NaOH (0.5 mL, 1.0 M), stir rapidly at 37 degrees, and react for 12 hours to obtain the following figure 1 (A) Au nanoclusters of 2-5 nm are shown.
[0027] Adding the synthesized gold nanoclusters to different concentrations of CuCl 2 solution, to obtain figure 1 (B) Fluorescence diagram shown. It can be found that gold nanoclusters can capture CuCl 2 , while CuCl 2 Can change AuNC s The optical properties of AuNC can be quenched under 365 nm UV light s of fluorescence.
Embodiment 2
[0028] Example 2: In vitro experiments show that gold nanoclusters reduce the cytotoxicity of copper ions:
[0029] Specifically, it includes two experiments:
[0030] The first group: study of different concentrations of CuCl 2 Cytotoxicity test
[0031] The steps are as follows:
[0032] 1) 96-well plates were plated, and 6000 GC1 cells were plated per well.
[0033] 2) Incubate for 24 hours.
[0034] 3) Using different concentrations of CuCl 2 Cells were treated for 24 hours.
[0035] 4) Add 10 μl of CCK8 reagent, place in a 37°C incubator, and measure the absorbance value at 450nm after 1 hour.
[0036] see figure 2 (A), when CuCl 2 The higher the concentration, the lower the survival rate of GC1 cells,
[0037] The second group: Studying different concentrations of AuNC s The reduction of CuCl 2 Cytotoxicity test
[0038] The specific steps are as follows:
[0039] 1) 96-well plates were plated, and 6000 GC1 cells were plated per well.
[0040] 2) Incubate ...
Embodiment 3
[0047] Example 3: Gold nanoclusters reduce CuCl 2 male reproductive toxicity.
[0048] The specific steps are as follows:
[0049] C57BL / 6N male mice were used.
[0050] Four groups of mice were treated as follows:
[0051] Group A: PBS group (3 mice): each mouse was intraperitoneally injected with 200 μl of PBS.
[0052] Group B: AuNC s Group (3 mice): Inject 200 μl of AuNC into the tail vein of each mouse s (18 mg / mL), and 200 μl AuNC was injected into the tail vein again after an interval of 1 hour s .
[0053] Group C: CuCl 2 Group (4 mice): Each mouse was intraperitoneally injected with CuCl according to body weight (4 μg / g) 2 solution.
[0054] Group D: CuCl 2 +AuNC sGroup (4 mice): Each mouse was intraperitoneally injected with CuCl according to body weight (4 μg / g) 2 solution. Immediately inject 200 μl AuNC into the tail vein s (18 mg / mL), and 200 μl AuNC was injected into the tail vein again after an interval of 1 hour s . After 48 hours, the mice in...
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