Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of gold nanoclusters and medicine for relieving male reproductive toxicity of copper ions

A gold nanocluster, male reproduction technology, applied in the field of biomedicine, can solve problems such as increasing excretion, and achieve the effects of improving absorption rate, reducing death, and enhancing targeting.

Active Publication Date: 2021-10-26
NANTONG UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Used to increase excretion or reduce absorption, but different metal chelation treatments have similar abnormal neurological deterioration, and hepatic copper metabolism disorders also require zinc salts to be used with caution, prompting the search for safer treatments

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of gold nanoclusters and medicine for relieving male reproductive toxicity of copper ions
  • Application of gold nanoclusters and medicine for relieving male reproductive toxicity of copper ions
  • Application of gold nanoclusters and medicine for relieving male reproductive toxicity of copper ions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Gold nanoclusters specifically absorb copper ions

[0025] Specific steps:

[0026] AuNCs were first synthesized: in one embodiment, chloroauric acid (5 mL, 10 mmol / L) and BSA (5 mL, 50 mg / mL) were stirred at 37°C for 2 minutes. Then add NaOH (0.5 mL, 1.0 M), stir rapidly at 37 degrees, and react for 12 hours to obtain the following figure 1 (A) Au nanoclusters of 2-5 nm are shown.

[0027] Adding the synthesized gold nanoclusters to different concentrations of CuCl 2 solution, to obtain figure 1 (B) Fluorescence diagram shown. It can be found that gold nanoclusters can capture CuCl 2 , while CuCl 2 Can change AuNC s The optical properties of AuNC can be quenched under 365 nm UV light s of fluorescence.

Embodiment 2

[0028] Example 2: In vitro experiments show that gold nanoclusters reduce the cytotoxicity of copper ions:

[0029] Specifically, it includes two experiments:

[0030] The first group: study of different concentrations of CuCl 2 Cytotoxicity test

[0031] The steps are as follows:

[0032] 1) 96-well plates were plated, and 6000 GC1 cells were plated per well.

[0033] 2) Incubate for 24 hours.

[0034] 3) Using different concentrations of CuCl 2 Cells were treated for 24 hours.

[0035] 4) Add 10 μl of CCK8 reagent, place in a 37°C incubator, and measure the absorbance value at 450nm after 1 hour.

[0036] see figure 2 (A), when CuCl 2 The higher the concentration, the lower the survival rate of GC1 cells,

[0037] The second group: Studying different concentrations of AuNC s The reduction of CuCl 2 Cytotoxicity test

[0038] The specific steps are as follows:

[0039] 1) 96-well plates were plated, and 6000 GC1 cells were plated per well.

[0040] 2) Incubate ...

Embodiment 3

[0047] Example 3: Gold nanoclusters reduce CuCl 2 male reproductive toxicity.

[0048] The specific steps are as follows:

[0049] C57BL / 6N male mice were used.

[0050] Four groups of mice were treated as follows:

[0051] Group A: PBS group (3 mice): each mouse was intraperitoneally injected with 200 μl of PBS.

[0052] Group B: AuNC s Group (3 mice): Inject 200 μl of AuNC into the tail vein of each mouse s (18 mg / mL), and 200 μl AuNC was injected into the tail vein again after an interval of 1 hour s .

[0053] Group C: CuCl 2 Group (4 mice): Each mouse was intraperitoneally injected with CuCl according to body weight (4 μg / g) 2 solution.

[0054] Group D: CuCl 2 +AuNC sGroup (4 mice): Each mouse was intraperitoneally injected with CuCl according to body weight (4 μg / g) 2 solution. Immediately inject 200 μl AuNC into the tail vein s (18 mg / mL), and 200 μl AuNC was injected into the tail vein again after an interval of 1 hour s . After 48 hours, the mice in...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides application of gold nanoclusters and a medicine for reducing male reproductive toxicity of copper ions, and relates to the technical field of biomedicine. The technical characteristic is that the gold nanoclusters are used for reducing cytotoxicity of the copper ions; the cell death is relieved; and the treatment on the testis copper metabolic disorder is realized, and the damage of copper ions to an organism is reduced.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of a gold nanocluster and a drug for alleviating the male reproductive toxicity of copper ions. Background technique [0002] Copper is an essential trace element for biological growth, the third most abundant trace element after iron and zinc, and plays an important role in maintaining the homeostasis of the body. Too much copper, however, can lead to oxidative damage to proteins and nucleic acids, lipid peroxidation, and enzyme inhibition. In the environment, copper pollution has become one of the heavy metal pollutants. It has been reported that chronic copper poisoning has a significant effect on the liver, which can lead to hepatolenticular degeneration and so on. There are also research reports that excessive copper will affect the reproductive system, reduce testosterone levels, reduce sperm motility and survival rate, reduce testicular weight, lead...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/242A61P15/08A61P39/02B82Y5/00
CPCA61K33/242A61P15/08A61P39/02B82Y5/00Y02A50/30
Inventor 李文清孙斐黄莹莹龚胜男
Owner NANTONG UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com