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Setaria viridis genetic conservative efficient in-vitro regeneration method

A technology of in vitro regeneration and green foxtail, which is applied in plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems of low germination rate and long time consumption of foxtail seeds, achieve high-efficiency in vitro regeneration, shorten germination time, The effect of improving the germination rate

Active Publication Date: 2021-10-29
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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Problems solved by technology

Therefore, with traditional planting methods, the germination rate of Setaria seeds is low and takes a long time (Ran Guangfu, Wu Lixian, Chen Yongbo, Luo Jiaxi, Luo Kejiang, Liu Zuwen, Xu Yanmei, Li Jiao, Xiong Mingbo, Zhou Yujing, Kuang Chongyi. African Setaria Research and application of rapid breeding technology [J]. Cao Xue, 2020(05):53-56.)

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  • Setaria viridis genetic conservative efficient in-vitro regeneration method
  • Setaria viridis genetic conservative efficient in-vitro regeneration method
  • Setaria viridis genetic conservative efficient in-vitro regeneration method

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Embodiment Construction

[0024] In the following, the present invention will be further described through specific embodiments, so as to better understand the present invention. But it does not constitute a limitation to the present invention.

[0025] (1) Preparation of explant material

[0026] Take mature foxtail seed ME34 as the initial explant, part of it is shelled, and the other part is not shelled. First, use 5‰ potassium permanganate solution for 2 minutes, then use 75% ethanol solution for 30 seconds, and finally use 10% NaClO The solution was treated for 10 minutes, and the sterilized seeds were inoculated into Murashige&Skoog (MS) liquid medium supplemented with 0, 1, 2, 3 mg / L 2,4-D, and the germination rate was counted after 15 days of liquid shallow shaking culture. Germinated seeds were inoculated into Murashige&Skoog (MS) solid medium to obtain initial explants ( figure 1 ).

[0027] The results show that shelling is more helpful for seed germination, and phytohormone 2,4-D can pro...

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Abstract

The invention discloses a setaria viridis genetic conservative efficient in-vitro regeneration method, which comprises the following steps: inoculating disinfected mature setaria viridis seeds into an MS liquid culture medium added with 2, 4-D, and performing liquid shallow layer shake culture to obtain sterile buds; performing direct adventitious bud induction culture and adventitious bud elongation culture; and carrying out rooting culture to obtain a complete regenerated plant. According to the method, the shortest time from single bud seedling cultivation to viable stable plant transplanting is only 35 days, and the genetic conservative property of setaria viridis can be kept.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for genetic conservation and high-efficiency in vitro regeneration of Setaria japonicus. Background technique [0002] Setaria viridis (L.) Beauv., also known as arhat grass, barnyard grass, is mainly distributed in temperate, tropical and subtropical regions. Setaria has strong resistance to salt-alkali and drought (Zhang Lanying, Chen Ruzhu, Li Gengguang. Setaria callus induction and plant regeneration[J]. Plant Physiology Communications, 1992(06):433), high yield, and also High-quality feed for herbivores (horses, cattle, sheep, etc.) provides guarantee for the development of animal husbandry. Setaria tropical C 4 Plants with short growth cycle and small genome (Zhao Hui, Zhang Lili, Guo Jingyuan, Huo Shanshan, He Pingping, Xia Qiyu, Fu Dongmei, Guo Anping. Study on drought resistance identification and evaluation index of model plant Setaria seed germination sta...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002A01H4/005
Inventor 朱木兰孙婧朗朱新广郑珂媛
Owner CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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