Holographic optical tweezer fusion structure illumination microscopic system and method

Abstract

The invention provides a holographic optical tweezers fusion structured illumination microscopic system and method, and belongs to the technical field of optical microsystems, the holographic optical tweezers fusion structured illumination microscopic system comprises a holographic optical tweezers micro-control system and a structured illumination microscopic system, the holographic optical tweezers micro-control system comprises a laser generating device, a light beam expanding unit and a light modulation unit; the light beam expanding unit comprises a first lens and a second lens which are sequentially arranged in the light emitting direction of the laser generating device, and the light modulation unit comprises a light spatial light modulator arranged in the light emitting direction of the second lens, and a third lens, a fourth lens, a first dichroic mirror and an objective lens which are sequentially arranged in the light emitting direction of the light spatial light modulator. And the structured illumination microscopic system comprises an illumination light source, and a structured light generator, a second dichroic mirror, a fifth lens, a first dichroic mirror and an objective lens which are sequentially arranged along the light emitting direction of the illumination light source.

Description

technical field [0001] The invention belongs to the technical field of optical microscopic systems, and relates to optical microscopic imaging and optical capture technology, in particular to a holographic optical tweezers fusion structured illumination microscopy system and method. Background technique [0002] Compared with the electron microscope, although the resolution of the optical microscope is relatively low, it is the best microscopic tool for studying living biological samples, especially in recent years, the rapid development of super-resolution optical imaging technology has improved the resolution of the optical microscope from hundreds of nanometers to a few nanometers. Therefore, optical microscopy is an important tool for studying microstructures, revealing life processes, and diagnosing pathology in the fields of biology and medicine. At present, the mainstream super-resolution optical imaging technology is mainly divided into two categories: one is the su...

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Problems solved by technology

[0003] In view of the above-mentioned problems in the prior art that the super-resolution and three-dimensional imaging optical microscopy of biological samples in the fluid solution cannot be realized, the present invention proposes a holographic optical tweezers fusion structure illumination microscopy system and method, which is fixed by optical tweezers For particles such as cells and macromolecules swimming in the solution, the incident light wavefront is modulated by a spatial light modulator, and the expected light field is obtained in the focal area of ​​the objective lens to capture and manipulate the particles, so as to realize super-resolution and three-dimensional imaging of biological samples in fluid solutions. Microscopic imaging

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