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Preparation method of dandelion extract and application thereof

A dandelion extract and dandelion technology are applied in the directions of medical preparations, drug combinations, and pharmaceutical formulations containing active ingredients, which can solve problems such as obstacles to research on dandelion, biological activity, and the relationship between dose-effect and structure-activity is not yet clear, and achieve inhibition of dandelion. Proliferation and cell migration, benefit maximization, efficacy in alleviating symptoms

Inactive Publication Date: 2021-11-02
JIANGSU OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the composition of dandelion extract is complex and various, and the relationship between the biological activity, dose-effect and structure-effect of each component is not yet clear, which hinders the research of dandelion in medicine

Method used

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  • Preparation method of dandelion extract and application thereof
  • Preparation method of dandelion extract and application thereof
  • Preparation method of dandelion extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The preparation of embodiment 1 dandelion water extract

[0048] Include the following steps:

[0049] (1) Weigh 5g of dandelion whole herb powder (passed through a 40 mesh sieve) and place it in a round bottom flask, add 15 times the amount of water and 12.5mL cellulase solution (final concentration 50U / mL, purchased from: Shanghai Macklin Biochemical Technology Co., Ltd., product number: C805042) After activation at 50°C for 30 minutes, decoct in a slight boiling state for 1 hour, filter out the decoction while it is hot, and collect it in a beaker. Stand at 4°C for 24 hours, filter, and concentrate the filtrate into an extract with a relative density of 1.1-1.15 (50°C) with a rotary evaporator, store it in a 4°C refrigerator for later use, and do three experiments in parallel.

[0050] (2) Determination of Chlorogenic Acid Content in Dandelion Water Extract

[0051] Preparation of the reference substance solution: Accurately weigh 6.493 mg of the chlorogenic acid r...

Embodiment 2

[0055] The purification of embodiment 2 dandelion extract

[0056] 1) D101 macroporous resin purification of dandelion extract

[0057] Extract the dandelion extract under the conditions of Example 1 and dilute it with distilled water at 1:10, and pass through the column in the D101 macroporous resin used. The ratio of the volume of the sample solution (mL) to the mass of the macroporous resin (g) is 12, pH=2 , the adsorption flow rate is 1.5BV / h, the desorption liquid ethanol concentration is 50%, the desorption flow rate is 2BV / h, and the desorption liquid consumption is 3BV. The separated liquid was collected in a conical flask, and concentrated under reduced pressure for use.

[0058] By HPLC, the contents of the organic acids of the three components before and after purification were compared. The content of three organic acids before purification is 71.91% ( Figure 9 ), which is 84.57% after purification.

[0059] 2) Determination of Chlorogenic Acid, Caffeic Acid a...

Embodiment 3

[0063] Example 3 Dandelion extract acts on rat granuloma experiment

[0064] The rats in this embodiment are SD rats with a body weight of 200-250 g, provided by the Experimental Animal Center of Nanjing University of Traditional Chinese Medicine.

[0065] The construction method of the rat model: After the rat was anesthetized with chloral hydrate, the underarm part was depilated and disinfected with 75% alcohol, and a 50 mg cotton ball soaked in 5 mL of 75% ethanol was implanted under the armpit of the rat's forelimb.

[0066] Rats were randomly divided into low, medium and high drug dose groups and model groups, a total of four groups, the doses of the three groups with low, medium and high drug concentrations were 1.56g crude drug / kg, 3.12g crude drug / kg, 6.24 g crude drug / kg, the model group was given normal saline. The rat model was continuously administered for 7 days, and 1 mL of drug was administered per 100 g of rat body weight. On the 8th day, the rats were killed ...

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Abstract

The invention provides a preparation method of a dandelion extract and the application of the dandelion extract in treatment or prevention of esophageal cancer. The preparation method comprises the following steps: (1) adding water and a cellulase solution into dandelion powder to activate, decocting, filtering out decoction, and concentrating into extract; and (2) diluting the extract obtained in the step (1), and concentrating and purifying by using a D101 macroporous resin column. According to the invention, the extraction rate and purification efficiency of organic acid are improved, and the dandelion extract has obvious prevention and treatment effects on inflammatory mice, and has good proliferation and cell migration inhibition capability on esophageal cancer cells.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to the application of dandelion and its extracts in the preparation of anti-esophagitis and esophageal cancer drugs. Background technique [0002] Esophageal cancer is a malignant tumor with a high mortality rate. There are many and complex pathogenic factors, which are mostly related to eating habits, obesity, gastroesophageal reflux disease, and Barrett's esophagus. Studies have found that the esophagus is often accompanied by the transformation of inflammatory cells and cytokines and the activation of inflammatory pathways in chronic inflammation, which transforms the microenvironment suitable for tumor proliferation and metastasis, and plays an important role in the process of inflammation-metagenesis-carcinogenesis. Therefore, clarifying the relationship between inflammation and the occurrence and development of esophageal cancer can provide new ideas fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/288A61P35/00A61P29/00A61P37/04A61P35/04
CPCA61K36/288A61P35/00A61P29/00A61P37/04A61P35/04A61K2236/19A61K2236/331A61K2236/55
Inventor 董自波王倩楠石锦峰王晗毓郑家勤李媛媛许锦涛
Owner JIANGSU OCEAN UNIV
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