Metal ion induced aspergillus oryzae gene promoter as well as preparation method and application thereof

Abstract

The invention provides a metal ion induced aspergillus oryzae gene promoter as well as a preparation method and application thereof. The nucleotide sequence of the aspergillus oryzae metal ion induced gene promoter is as shown in SEQ ID NO:1. The promoter is induced to express by zinc and manganese ions. Under the induction concentration of zinc and manganese ions, the zinc and manganese ions do not inhibit the growth of aspergillus oryzae, but promote the growth of aspergillus oryzae. Moreover, the inducers ZnSO4 and MnSO4 are low in price, so that wide application in industrial production is facilitated.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a metal ion-induced aspergillus oryzae gene promoter, a preparation method and application thereof. Background technique [0002] Aspergillus oryzae is a filamentous fungus of great value in industrial production, and is widely used in food, enzyme preparation, medicine and cosmetic industries. In order to obtain high-yielding and superior-performance Aspergillus oryzae strains, researchers have developed a series of molecular genetic manipulation tools for Aspergillus oryzae, including homologous recombination, gene knockout CRISPR system, RNA interference technology, etc. However, the development of these molecular genetic manipulation technologies is inseparable from the development of gene expression regulatory elements, such as the mining of inducible promoters. [0003] Compared with the relatively complete gene expression regulation tool library of bacteria an...

AI Technical Summary

Problems solved by technology

For carbon source-dependent inducible promoters, phenomena such as glucose repression may affect product synthesis due to carbon source limitation

For metabolite-dependent inducible promoters, due to the occurrence of metabolite feedback inhibition, the growth of the microorganism itself may be inhibited, thereby affecting the yield of the product, and, for thiA promoters, vitamin B1 often inhibits the thiA promoter The effect is not obvious, thus losing its role in regulating gene expression

In addition, inducers are not cheap enough

Images