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Wheat mutation breeding method
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A technology for mutation breeding and wheat, applied in the directions of botanical equipment and methods, application, and plant genetic improvement, can solve the problems of high cost, low mutagenesis rate, long construction time, etc., and achieve economical convenience and good mutagenesis effect. , the effect of speeding up
Inactive Publication Date: 2021-11-09
CROP SCI RES INST SHANXI ACADEMY OF AGRI SCI
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[0002] The radiation mutagenesis currently used requires equipment such as radiation sources and protective devices, which is expensive, takes a long time to construct, needs to deal with radioactive substances, and has a low mutagenesis rate
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Embodiment 1
[0026] see figure 1 , the present invention provides a kind of technical scheme: a kind of wheat mutation breeding method, concrete steps are as follows:
[0027] S1. Preparation of buffer solution: prepare acetate buffer solution;
[0028] S2, preparation of mutagen: mix the prepared buffer solution with nitrous acid methyl urea solution to make a mutagen of pH7;
[0029] S3, soaking mutagenesis: putting the wheat seeds into a petri dish containing a mutagen and soaking;
[0030] S4, post-treatment: rinse the soaked wheat seeds with a cleaning solution to remove the mutagen, and the treatment time is not less than 48 hours;
[0031] S5. Breeding: transplant the treated wheat seeds into soil for planting.
[0032] In step S1, ammonium acetate, hydrochloric acid and ammonia water were used to prepare a 0.2 mol / L buffer solution with a pH of 4.5, and then distilled water was added to dilute the buffer solution to a 0.1 mol / L solution.
[0033] In step S2, dilute the nitrous ...
Embodiment 2
[0043] see figure 1 , the present invention provides a kind of technical scheme: a kind of wheat mutation breeding method, concrete steps are as follows:
[0044] S1. Preparation of buffer solution: prepare acetate buffer solution;
[0045] S2, preparation of mutagen: mix the prepared buffer solution with nitrous acid methyl urea solution to make a mutagen of pH7;
[0046] S3, soaking mutagenesis: putting the wheat seeds into a petri dish containing a mutagen and soaking;
[0047] S4, post-treatment: rinse the soaked wheat seeds with a cleaning solution to remove the mutagen, and the treatment time is not less than 48 hours;
[0048] S5. Breeding: transplant the treated wheat seeds into soil for planting.
[0049] In the step S1, ammonium acetate, hydrochloric acid and ammonia water are used to prepare a 0.2 mol / L buffer solution with a pH of 4.5, and then distilled water is added to dilute the buffer solution to a 0.1 mol / L solution.
[0050] In the step S2, dilute the ni...
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Abstract
The invention discloses a wheat mutation breeding method. The method comprises the following specific steps: S1, preparation of a buffer solution: preparing an acetate buffer solution; S2, preparation of a mutagenic agent: mixing the prepared buffer solution with an organic methylurea nitrite solution to prepare the mutagenic agent with the pH value of 7; S3, soaking mutagenesis: putting the wheat seeds into a culture dish containing the mutagenic agent to be soaked; S4, aftertreatment: washing the soaked wheat seeds with a cleaning solution, and removing the mutagenic agent, wherein the treatment time is not shorter than 48 H; S5, breeding: transplanting the treated wheat seeds into soil for planting. According to the method, the seeds are soaked in the mutagenic agent, the mutagenic agent enters embryonic cells through low-temperature treatment, then the seeds are transferred into the fresh mutagenic agent, and the mutagenesisreaction speed is increased.
Description
technical field [0001] The invention relates to the technical field of mutation breeding, in particular to a wheat mutation breeding method. Background technique [0002] The radiationmutagenesis currently used requires equipment such as radiation sources and protective devices, which is expensive, takes a long time to construct, needs to deal with radioactive substances, and has a low mutagenesis rate. Contents of the invention [0003] The purpose of the present invention is to provide a wheat mutation breeding method to solve the problems raised in the above background technology. [0004] In order to achieve the above object, the present invention provides the following technical solutions: a wheat mutation breeding method, the specific steps are as follows: [0005] S1. Preparation of buffer solution: prepare acetate buffer solution; [0006] S2, preparation of mutagen: mix the prepared buffer solution with nitrous acid methyl urea solution to make a mutagen of pH7...
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IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 任永康
Owner CROP SCI RES INST SHANXI ACADEMY OF AGRI SCI
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