Molecular marker primer combination and its application for rapid identification of super-large fruit shape traits of Myrica rubra
A combination of primers and molecular marker technology, applied in the field of molecular biology, can solve the problems of delayed bayberry fruit type directional breeding process, fruit size and shape are not yet known, achieve high throughput, high sensitivity and accuracy, and short PCR reaction time , the effect of strong stability
Active Publication Date: 2022-04-05
NINGBO ACAD OF AGRI SCI
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Problems solved by technology
[0004] However, due to the long childhood period of red bayberry (more than 8 years after seed propagation and planting), the size and shape of its fruit are unknown and there are no other characteristics that can clearly indicate the fruit size and shape of its adult stage, which seriously lags behind the process of directional breeding of red bayberry fruit type
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Embodiment 1
[0029] A set of molecular marker primer combinations and applications for identifying super-large fruit-type traits of Myrica rubra, the detailed steps are:
[0030] (1) Primer synthesis: according to the sequence information in Table 1, entrust Sangon Bioengineering (Shanghai) Co., Ltd. to synthesize primer BS16-25856;
[0031] Table 1: BS16-25856 primer sequence information
[0032]
[0033] (2) Extraction of leaf DNA of samples of bayberry germplasm resources to be tested:
[0034] A. Grind about 0.2 g of red bayberry young leaves into powder and place them in a 2 mL centrifuge tube with liquid nitrogen;
[0035] B. Use the Ezup column plant genomic DNA extraction kit (EZ-10Spin Column Plant GenomicDNA Purification Kit, Shanghai Sangon Bioengineering Co., Ltd.) to extract the genomic DNA of the above samples, and the specific operation steps are carried out according to its instructions;
[0036] C. Take 1-2 μl for detection on 1.0% agarose gel, dilute the DNA stock so...
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The invention discloses a group of molecular marker primer combinations capable of quickly identifying super-large fruit shape traits of bayberry and its application, with a view to being applied to the early auxiliary selection of the size and shape traits of bayberry fruit. The invention provides a set of molecular marker primer combinations that can quickly identify the super-large fruit shape of bayberry fruit, which is BS16-25856, and is suitable for high-throughput typing detection platforms such as fluorescent quantitative PCR instruments. The amplified fragment is short, time-saving and sensitive. high. Based on the developed molecular marker primer combination, the invention provides a method for assisting in the screening of excellent bayberry germplasm resources, which has important guiding significance in the prediction of bayberry fruit morphological traits, phenotypes and hybrid breeding, and has the advantages of simple operation, high efficiency, fast speed and low cost Etc.
Description
technical field [0001] The invention relates to a group of molecular marker primer combinations and application thereof for rapidly identifying super-large fruit type characters of red bayberry, belonging to the field of molecular biology. Background technique [0002] The rapid development of modern agriculture has led to the transformation of people's consumption patterns towards high quality, diversification and diversification, and has put forward higher and higher requirements for the fruit size and internal quality of red bayberry. Therefore, one of the main goals of current bayberry breeding should be established with large fruit size, high quality, stable yield, and strong storage and transportation resistance. [0003] Red bayberry (Myrica rubra Sieb. and Zucc.) is native to my country and belongs to one of the eight precious fruits in the south of the Yangtze River. Its fruit is rich in anthocyanins, has high nutritional value, and is suitable for both fresh food ...
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IPC IPC(8): C12Q1/6895C12Q1/686C12N15/11
CPCC12Q1/6895C12Q1/686C12Q2600/13C12Q2600/156C12Q2563/107C12Q2545/114
Inventor 焦云房聪玲柴春燕高中山汪国云贾慧敏舒巧云周超超
Owner NINGBO ACAD OF AGRI SCI