Anti-HPV (human papillomavirus) polyphenol slow-release lipid nanoparticle gel and preparation method thereof

A lipid nanoparticle and HPV virus technology, applied in the field of medicine, can solve problems such as the formulation and process content of solid lipid nanoparticle gel that does not have sustained release and does not involve polyphenol sustained release

Pending Publication Date: 2021-11-12
SHENYANG WANJIA INST OF BIOLOGICAL TECH RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent 201810027344.X, a tea polyphenol composition, discloses a prescription and preparation method of a tea polyphenol composition. The patent involves the formulation and preparation method of ointment and hydrogel patch, mainly for external skin use. Does not have sustained release effects
[0006] None of the above patents, documents and marketed products involve polyphenol sustained-release solid lipid nanoparticle gel formula and process content

Method used

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  • Anti-HPV (human papillomavirus) polyphenol slow-release lipid nanoparticle gel and preparation method thereof
  • Anti-HPV (human papillomavirus) polyphenol slow-release lipid nanoparticle gel and preparation method thereof
  • Anti-HPV (human papillomavirus) polyphenol slow-release lipid nanoparticle gel and preparation method thereof

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Embodiment 1

[0023] The preparation method of the anti-HPV virus polyphenol sustained-release lipid nanoparticle gel of the present invention is as follows: a. Get 1 weight part of Carbomer 974P and 2 weight parts of polycarbophil, add 100 parts by weight of water, and stir at room temperature for 2 hours , to get a hydrated gel, adjust the pH to 3.3 with a 10% sodium hydroxide aqueous solution with a mass percentage concentration, and get a gel for subsequent use; b. get 1 weight part of tea polyphenols, 1 weight part of glyceryl monostearate, 1 weight part Add 15 parts by weight of absolute ethanol to 1 part by weight of soybean lecithin, mix and melt under the condition of raising the temperature in a water bath to 70°C, heat and recover the ethanol in a vacuum, and obtain the lipid oil phase, take 1 part by weight of poloxamer 188, add 35 parts by weight of purified water, Obtain the water phase and raise the temperature to 70°C, mix the two phases for high-shear homogenization for 2 mi...

Embodiment 2

[0025] The preparation method of the anti-HPV virus polyphenol slow-release lipid nanoparticle gel of the present invention is as follows: a. Get 2 parts by weight of Carbomer 974 and 3 parts by weight of polycarbophil, add 110 parts by weight of water, and stir at room temperature for 2.5 hours , to obtain a hydrated gel, adjust the pH to 3.3 with a 10% aqueous solution of sodium hydroxide with a concentration of 10% by mass, and obtain a gel for subsequent use; b. get 1 part by weight of resveratrol, 2 parts by weight of mixed glycerides, 1 part by weight of Soybean lecithin, add 18 parts by weight of absolute ethanol, mix and melt under the condition of raising the temperature to 75°C in a water bath, heat and recover the ethanol in a vacuum, and obtain the lipid oil phase, take 1 part by weight of poloxamer 407, add 30 parts by weight of purified water, and obtain The water phase was heated to 75°C, and the two phases were mixed and homogenized under high shear for 4 minute...

Embodiment 3

[0027] The preparation method of the anti-HPV virus polyphenol slow-release lipid nanoparticle gel of the present invention is as follows: a. Get 3 parts by weight of Carbomer 974P and 4 parts by weight of polycarbophil, add 120 parts by weight of water, and stir at room temperature for 3 hours , to obtain a hydrated gel, adjust the pH to 3.3 with a 10% aqueous sodium hydroxide solution with a concentration of 10% by mass, and obtain a gel for subsequent use; Phospholipids, adding 20 parts by weight of absolute ethanol, mixing and melting in a water bath to 70°C, heating and recovering the ethanol in a vacuum to obtain a lipid oil phase, taking 1 part by weight of Poloxamer 188 and 1 part by weight of Poloxamer 407, Add 40 parts by weight of purified water to obtain an aqueous phase and raise the temperature to 70°C, mix the two phases for high-shear homogenization for 6 minutes, and obtain a lipid nanoparticle solution for later use; c. heat the gel obtained in a to 60°C, add ...

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Abstract

The invention discloses an anti-HPV (human papilloma virus) polyphenol sustained-release lipid nanoparticle gel and a preparation method thereof, the preparation is mainly used for vaginal administration, mucous membrane administration or skin administration, is used for inhibiting papilloma virus infection, and belongs to the technical field of medicines. The invention relates to an anti-HPV (human papillomavirus) polyphenol sustained-release lipid nanoparticle gel, which is prepared from the following raw materials in parts by weight: 1 part of polyphenol, 1-5 parts of lipid component, 1-3 parts of soybean phospholipid, 1-3 parts of poloxamer, 2-6 parts of polycarbophil, 1-3 parts of carbomer, 5-10 parts of liquid paraffin and 22-28 parts of glycerol. The anti-HPV (human papilloma virus) polyphenol sustained-release lipid nanoparticle gel has the advantages that polyphenol medicine components can be slowly released, medicine adhesion is kept without flowing out, a certain medicine concentration is kept in a local environment and mucous membranes or skin layers, and the effect of inhibiting HPV viruses and infected cells is improved.

Description

technical field [0001] The present invention relates to an anti-HPV virus drug for vaginal administration, mucous membrane or skin administration, in particular to an anti-HPV virus polyphenol slow-release lipid nanoparticle gel for local treatment and prevention of human nipple The invention relates to warts and cervical cancer diseases caused by tumor virus HPV, belonging to the technical field of medicine. Background technique [0002] HPV mainly infects human skin epithelium and mucosal epithelium. HPVE1 and E2 proteins regulate the replication and transcription of the viral genome, E4 protein assists virus maturation, and high-risk HPVE5, E6, and E7 are the main factors that cause cancer. Immortalization plays a decisive role. The persistent infection of HPV virus can lead to the integration of HPV DNA into human cells. The integration of HPV DNA often results in the deletion of E1 and E2 regions in cervical cells, which in turn leads to the persistent high expression ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K47/32A61K47/14A61K47/10A61K9/127A61K31/05A61K31/12A61K31/353A61K47/06A61K47/26A61P15/00A61P15/02A61P17/12A61P31/20A61P35/00B82Y5/00B82Y40/00
CPCA61K31/05A61K31/353A61K31/12A61K9/127A61K47/14A61K47/24A61K47/26A61K9/06A61K47/32A61K47/06A61K9/0034A61K9/0014A61K47/10A61P31/20A61P35/00A61P15/02A61P15/00A61P17/12B82Y5/00B82Y40/00
Inventor 董英杰艾莉李晓怡邹晓峰韩亚男丁爽
Owner SHENYANG WANJIA INST OF BIOLOGICAL TECH RES
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