Multipath fluorescence detection system, device and method

Abstract

The invention provides a multipath fluorescence detection system, device and method; the system is provided with an excitation light source, a light filter group, an imaging lens group and a CMOS sensor which are sequentially arranged from an object end to an image end along an optical axis; the excitation light source comprises LED light sources with a plurality of wavelengths; the light filter group comprises at least one fan-shaped light filtering area; the number of the fan-shaped light filtering areas is consistent with the number of the wavelengths of the LED light sources; the excitation light source is used for outputting excitation light through the LED light source and performing uniform exposure on a to-be-detected object, so that the to-be-detected object generates fluorescent; and the light filter group is used for transmitting target wavelength fluorescence and cutting off non-target wavelength fluorescence. According to the technical scheme in the invention, in the detection process, fluorescence of the to-be-detected object is excited through the LED light sources; through the fan-shaped areas of light filters, non-target wavelength fluorescence is cut off; target wavelength fluorescence is reserved; efficient wavelength conversion and fluorescence quantitative detection can be conveniently and rapidly achieved by rapidly switching on and off of LEDs; and the invention can be widely applied to the technical field of biology.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a multi-channel fluorescence detection system, device and detection method. Background technique [0002] PCR (Polymerase Chain Reaction, Polymerase Chain Reaction) is a molecular biology technique used to amplify specific DNA fragments. It can be regarded as special DNA replication outside the body. The biggest feature of PCR is that it can With the development of science and technology, PCR technology is increasingly being used in archaeology, criminal investigation and other fields related to biological science and technology. [0003] Real-time fluorescent quantitative PCR is the mainstream tool for life science research at present. Compared with traditional PCR, real-time fluorescent quantitative PCR adds an optical detection system on the basis of ordinary PCR. The emitted light signal of the DNA fragment increases accordingly during the amplification process, an...

AI Technical Summary

Problems solved by technology

Regardless of which of the above methods is used, to realize the detection of multiple fluorescent channels, it is necessary to switch the filter set, and a mechanical device is required to realize the conversion of the filter position, which has poor stability and further prolongs the detection time.

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