Multipath fluorescence detection system, device and detection method

Abstract

The invention provides a multipath fluorescence detection system, device and detection method. The system is provided with an excitation light source, an optical filter group, an imaging lens group and a CMOS sensor which are sequentially arranged from an object end to an image end along an optical axis; the excitation light source comprises LED light sources with a plurality of wavelengths, the light filter group comprises at least one fan-shaped light filtering area, and the number of the fan-shaped light filtering areas is consistent with the number of the wavelengths of the LED light sources; the excitation light source is used for outputting excitation light through the LED light source and performing uniform exposure on a to-be-detected object, so that the to-be-detected object generates fluorescent light; the optical filter group is used for transmitting target wavelength fluorescence and cutting off non-target wavelength fluorescence; according to the technical scheme, in the detection process, fluorescence of the object to be detected is excited through the LED light sources, non-target wavelength fluorescence is cut off through the sectors of the optical filters, target wavelength fluorescence is reserved, efficient wavelength conversion and fluorescence quantitative detection can be conveniently and rapidly achieved by rapidly switching on and off of the LEDs, and the detection accuracy is high. The method can be widely applied to the technical field of biology.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a multiplex fluorescence detection system, device and detection method. Background technique [0002] PCR (Polymerase Chain Reaction) is a molecular biology technique used to amplify specific DNA fragments. It can be regarded as a special DNA replication in vitro. The biggest feature of PCR is that it can With the development of science and technology, PCR technology is increasingly used in archaeology, criminal investigation and other fields related to biological science and technology. [0003] Real-time quantitative PCR is the mainstream tool for life science research. Compared with traditional PCR, real-time quantitative PCR adds an optical detection system on the basis of ordinary PCR. The emitted light signal of the DNA fragment increases during the amplification process, and the content of the original sample can be calculated by detecting the intensity of the e...

AI Technical Summary

Problems solved by technology

Regardless of which of the above methods is used, to realize the detection of multiple fluorescent channels, it is necessary to switch the filter set, and a mechanical device is required to realize the conversion of the filter position, which has poor stability and further prolongs the detection time.

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