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Method for culturing animal-origin-free umbilical cord mesenchymal stem cells

A technology of stem cells and culture methods, applied in animal cells, vertebrate cells, cell culture supports/coatings, etc., can solve problems such as waste of manpower and material resources, unqualified stem cell phenotype, and device use

Pending Publication Date: 2021-11-16
辽宁医学诊疗科技研发中心有限公司
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AI Technical Summary

Problems solved by technology

In the past traditional cell biology preparation method, the prepared stem cells were used due to unqualified cell phenotype, which wasted a lot of manpower and material resources. The present invention solves this problem to a certain extent

Method used

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  • Method for culturing animal-origin-free umbilical cord mesenchymal stem cells
  • Method for culturing animal-origin-free umbilical cord mesenchymal stem cells
  • Method for culturing animal-origin-free umbilical cord mesenchymal stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. The package is proportional to the substrate: 1: 50. Four T75 culture bottles were taken with 100 ul Stemcell base gel (# 07130) and 4.9 mL PBS. The package is 3 hours after gentle mixing.

[0036] 2, the treatment of umbilical cord: Operation in the GMP Lab, Shanghai Lishen Science Instrument Co., Ltd. Biosafety Cabinet (HFSAFE1200LC), fresh and healthy umbilical cord, total length 25cm, take 1cm long, washed once with 75% alcohol, 0.9% chlorine The sodium sodium injection is rinsed twice to remove the residual blood; cutting the umbilical cord along the umbilical vein, uses the blood clamp and scissors to remove 1 umbilical vein, 2 risolding arteries, rinsed with 0.9% sodium chloride injection twice, with scissors shear Broken into 1mm 3 The large group of woven blocks were used to apply a 5 mL concentration of 2 mg / L Solaibao collaboration. After chemistry, CD105 expressed 98% of CD105 to be qualified umbilical cords, carry out the next step;

[0037] 2) The above u...

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Abstract

The invention relates to the field of medical treatment, and provides a culture method of umbilical cord mesenchymal stem cells without animal origin in order to overcome the defects of an existing mesenchymal stem cell culture method. The umbilical cord mesenchymal stem cell culture method comprises the following steps: 1, coating matrigel; 2, umbilical cord tissue separation; 3, changing the liquid; 4, treating primary cells, and carrying out cell passage; and 5, cryopreserving the cells.

Description

Technical field [0001] The present invention relates to the field of medical fields, in particular a culture process for umbilical trunk mesenchymal stem cells. Background technique [0002] In recent years, with the rapid development of life sciences and medicine, domestic and foreign innovative biotherapy technology has progressed rapidly, and cellular treatment has developed into a third medical revolution after drug treatment and surgical treatment, in malignant tumors, inflammation, autoimmune diseases. , Anti-aging, regenerative medicine and other fields show huge application potential. Cellotherapy includes immunocyte treatment and stem cell therapy, stem cell therapy including embryonic stem cells (ESC), tissue-specific precursor stem cells (TSPSC), mesenchymal stem cells (MSC), umbilical cord stem cells (UCSC), bone marrow stem cells (BMSC) and induced Multi-potential stem cells (IPSC), etc. At present, stem cells have become an indispensable effective means of renewing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A01N1/02
CPCC12N5/0665A01N1/02C12N2533/90
Inventor 张长青
Owner 辽宁医学诊疗科技研发中心有限公司
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